Collagen XVI Induces Expression of MMP9 via Modulation of AP-1 Transcription Factors and Facilitates Invasion of Oral Squamous Cell Carcinoma
| العنوان: | Collagen XVI Induces Expression of MMP9 via Modulation of AP-1 Transcription Factors and Facilitates Invasion of Oral Squamous Cell Carcinoma |
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| المؤلفون: | Konstanze B Bedal, Susanne Grässel, Peter J Oefner, Joerg Reinders, Torsten E Reichert, Richard Bauer |
| المصدر: | PLoS ONE PLoS ONE, Vol 9, Iss 1, p e86777 (2014) |
| بيانات النشر: | Public Library of Science, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Proteomics, Oral Medicine, Blotting, Western, 610 Medizin, lcsh:Medicine, Apoptosis, Protein Serine-Threonine Kinases, Real-Time Polymerase Chain Reaction, Head and Neck Squamous Cell Carcinoma, Cell Movement, Molecular Cell Biology, Cell Adhesion, Tumor Cells, Cultured, Humans, Immunoprecipitation, Neoplasm Invasiveness, RNA, Messenger, lcsh:Science, Promoter Regions, Genetic, Biology, Cell Proliferation, Oncogenic Signaling, ddc:610, Spectrometric Identification of Proteins, Reverse Transcriptase Polymerase Chain Reaction, lcsh:R, Cancers and Neoplasms, Membrane Proteins, Signaling in Selected Disciplines, Head and Neck Tumors, Neoplasm Proteins, Transcription Factor AP-1, Oncology, Otorhinolaryngology, Head and Neck Cancers, Matrix Metalloproteinase 9, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Carcinoma, Squamous Cell, Medicine, lcsh:Q, Mouth Neoplasms, Collagen, Research Article, Signal Transduction |
| الوصف: | Collagen XVI belongs to the family of fibril-associated collagens with interrupted triple helices (FACIT). It is overexpressed during the progression of oral squamous cell carcinoma (OSCC). The present data show a strong collagen XVI-dependent induction of MMP9 and an increase in OSCC cell invasion. We found activated integrin-linked kinase (ILK) in a complex with kindlin-1 and activation of protein kinase B (PKB/Akt) to be responsible for MMP9 induction. Inhibition of the formation of focal adhesions reduced MMP9 expression. Moreover, collagen XVI overexpressing OSCC cell clones (COLXVI cell clones) transfected with vectors containing different MMP9 promoter fragments adjacent to a luciferase reporter revealed an increase in luciferase signal dependent on AP-1 binding sites. Deletion of the AP-1 binding site 98 bp upstream of the reported transcription start site and inhibition of AP-1 with Tanshinone IIA resulted in decreased MMP9 expression. The AP-1 subunit JunB showed differential expression between COLXVI cell clones and mock control cells. Additionally, mass spectrometric analysis of immunoprecipitates revealed that c-Fos interacted strongly with dyskerin in COLXVI cell clones compared to mock controls. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a1ab41c02f4dade2067fca71f7183acTest http://europepmc.org/articles/PMC3900656Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0a1ab41c02f4dade2067fca71f7183ac |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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