The effect of standoff distance and surface roughness on biofilm disruption using cavitation
| العنوان: | The effect of standoff distance and surface roughness on biofilm disruption using cavitation |
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| المؤلفون: | Victoria Franke Stenport, Sarah A. Kuehne, Rachel Sammons, Qianxi Wang, Anthony Walmsley, Cecilia Johansson, Nina Vyas |
| المصدر: | PLoS ONE PLoS ONE, Vol 15, Iss 7, p e0236428 (2020) |
| بيانات النشر: | Public Library of Science, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Time Factors, Medical Implants, Scanning electron microscope, Diagnostic Radiology, 0302 clinical medicine, Ultrasound Imaging, Surface roughness, Medicine and Health Sciences, Image Processing, Computer-Assisted, Electron Microscopy, Ultrasonics, Composite material, Titanium, Microscopy, Multidisciplinary, Radiology and Imaging, Biomaterial, Chemistry, Cavitation, Physical Sciences, Medicine, Engineering and Technology, Scanning Electron Microscopy, Research Article, Biotechnology, Chemical Elements, Materials science, Imaging Techniques, Surface Properties, Science, Biomaterial Implants, chemistry.chemical_element, Bioengineering, Image Analysis, Research and Analysis Methods, Microbiology, 03 medical and health sciences, Diagnostic Medicine, Dental ultrasonic scaler, Titanium Implants, Dental Implants, Biofilm, Biology and Life Sciences, Ultrasonic scaler, Bacteriology, 030206 dentistry, biochemical phenomena, metabolism, and nutrition, 030104 developmental biology, chemistry, Biofilms, Medical Devices and Equipment, Bacterial Biofilms |
| الوصف: | Effective biofilm removal from surfaces in the mouth is a clinical challenge. Cavitation bubbles generated around a dental ultrasonic scaler are being investigated as a method to remove biofilms effectively. It is not known how parameters such as surface roughness and instrument distance from biofilm affect the removal. We grew Strepotococcus sanguinis biofilms on coverslips and titanium discs with varying surface roughness (between 0.02-3.15 μm). Experimental studies were carried out for the biofilm removal using high speed imaging and image analysis to calculate the area of biofilm removed at varying ultrasonic scaler standoff distances from the biofilm. We found that surface roughness up to 2 μm does not adversely affect biofilm removal but a surface roughness of 3 μm caused less biofilm removal. The standoff distance also has different effects depending on the surface roughness but overall a distance of 1 mm is just as effective as a distance of 0.5 mm. The results show significant biofilm removal due to an ultrasonic scaler tip operating for only 2s versus 15-60s in previous studies. The technique developed for high speed imaging and image analysis of biofilm removal can be used to investigate physical biofilm disruption from biomaterial surfaces in other fields. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3218e3d2ed3ebc1f556e102d48c975cTest http://europepmc.org/articles/PMC7392287Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c3218e3d2ed3ebc1f556e102d48c975c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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