Colonization of the cervicovaginal space with Gardnerella vaginalis leads to local inflammation and cervical remodeling in pregnant mice
| العنوان: | Colonization of the cervicovaginal space with Gardnerella vaginalis leads to local inflammation and cervical remodeling in pregnant mice |
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| المؤلفون: | Snehal S. Shetye, Guillermo Barila, Lauren Anton, Michal A. Elovitz, Carrie E. Barnum, Louis J. Soslowsky, Amy Brown, Luz-Jeannette Sierra |
| المصدر: | PLoS ONE PLoS ONE, Vol 13, Iss 1, p e0191524 (2018) |
| بيانات النشر: | Public Library of Science, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Amniotic fluid, Maternal Health, Uterus, lcsh:Medicine, Artificial Gene Amplification and Extension, Cervix Uteri, medicine.disease_cause, Pathology and Laboratory Medicine, Biochemistry, Polymerase Chain Reaction, Cervix, Mice, 0302 clinical medicine, Pregnancy, Medicine and Health Sciences, Gardnerella vaginalis, lcsh:Science, Immune Response, 030219 obstetrics & reproductive medicine, Multidisciplinary, Obstetrics and Gynecology, Animal Models, Vaginosis, Bacterial, 3. Good health, Biomechanical Phenomena, medicine.anatomical_structure, Real-time polymerase chain reaction, Experimental Organism Systems, Vagina, Cytokines, Female, Bacterial vaginosis, Anatomy, Genital Anatomy, Research Article, Immunology, Mouse Models, Enzyme-Linked Immunosorbent Assay, Research and Analysis Methods, Preterm Birth, Real-Time Polymerase Chain Reaction, Andrology, 03 medical and health sciences, Model Organisms, Signs and Symptoms, Diagnostic Medicine, Placenta, medicine, Animals, Molecular Biology Techniques, Molecular Biology, Inflammation, Fetus, business.industry, lcsh:R, Reproductive System, Biology and Life Sciences, Proteins, medicine.disease, Pregnancy Complications, 030104 developmental biology, Birth, Women's Health, lcsh:Q, business, Collagens |
| الوصف: | The role of the cervicovaginal (CV) microbiome in regulating cervical function during pregnancy is poorly understood. Gardnerella vaginalis (G. vaginalis) is the most common bacteria associated with the diagnosis of bacterial vaginosis (BV). While BV has been associated with preterm birth (PTB), clinical trials targeting BV do not decrease PTB rates. It remains unknown if G. vaginalis is capable of triggering molecular, biomechanical and cellular events that could lead to PTB. The objective of this study was to determine if cervicovaginal colonization with G. vaginalis, in pregnant mice, induced cervical remodeling and modified cervical function. CD-1 timed-pregnant mice received a 5X108 CFU/mL intravaginal inoculation of G. vaginalis or control on embryonic day 12 (E12) and E13. On E15, the mice were sacrificed and cervicovaginal fluid (CVF), amniotic fluid (AF), cervix, uterus, placentas and fetal membranes (FM) were collected. Genomic DNA was isolated from the CVF, placenta, uterus and FM and QPCR was performed to confirm colonization. IL-6 was measured in the CVF and AF and soluble e-cadherin (seCAD) was assessed in the CVF by ELISA. RNA was extracted from the cervices to evaluate IL-10, IL-8, IL-1β, TNF-α, Tff-1, SPINK-5, HAS-1 and LOX expression via QPCR. Mucicarmine and trichrome staining was used to assess cervical mucin and collagen. Biomechanical properties of the cervix were studied using quasi-static tensile load-to-failure biomechanical tests. G. vaginalis successfully colonized the CV space. This colonization induced immune responses (increased IL-6 levels in CVF and AF, increased mRNA expression of cervical cytokines), altered the epithelial barrier (increased seCAD in the CVF), induced cervical remodeling (increased mucin production, altered collagen) and altered cervical biomechanical properties (a decrease in biomechanical modulus and an increase in maximum strain). The ability of G. vaginalis to induce these molecular, immune, cellular and biomechanical changes suggests that this bacterium may play a pathogenic role in premature cervical remodeling leading to PTB. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::325fa8864192193361b1ae6f9dbe01dcTest http://europepmc.org/articles/PMC5773211Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....325fa8864192193361b1ae6f9dbe01dc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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