Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4
| العنوان: | Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4 |
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| المؤلفون: | Hui Chen, Yiguo Chen, Wei-Ping Min, Xiao-Mu Wu, Linqiang Deng, Keng Yuan, Yujuan Zhang, Lizhi Yu, Yong Zhang, Zhanghua Xiong, Yan-Rong Yu, Nanjin Zhou, Xiaomei Gui |
| المصدر: | PLoS ONE PLoS ONE, Vol 11, Iss 3, p e0149233 (2016) |
| بيانات النشر: | Public Library of Science, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Pulmonology, Neutrophils, Physiology, Staphylococcus, Respiratory System, lcsh:Medicine, Complement receptor, medicine.disease_cause, Immune Receptors, Biochemistry, White Blood Cells, Mice, Animal Cells, Immune Physiology, Pneumonia, Staphylococcal, Medicine and Health Sciences, lcsh:Science, Receptor, Immune Response, Toll-like Receptors, Pathology and laboratory medicine, Innate Immune System, Multidisciplinary, Immune System Proteins, biology, Lactoferrin, Animal Models, Medical microbiology, Staphylococcus aureus, Cytokines, Methicillin-resistant Staphylococcus aureus, Cellular Types, Pathogens, Anatomy, Research Article, Signal Transduction, Agonist, medicine.drug_class, Immune Cells, 030106 microbiology, Immunology, Macrophage-1 Antigen, Bronchi, Mouse Models, Research and Analysis Methods, Microbiology, 03 medical and health sciences, Lipopeptides, Signs and Symptoms, Model Organisms, medicine, Animals, Inflammation, Innate immune system, Blood Cells, Bacteria, lcsh:R, Receptors, IgG, Organisms, Biology and Life Sciences, Proteins, Cell Biology, Pneumonia, Molecular Development, Toll-Like Receptor 2, Microbial pathogens, TLR2, 030104 developmental biology, Immune System, biology.protein, Receptors, Complement 3b, lcsh:Q, Bacterial pathogens, Developmental Biology |
| الوصف: | The spread of methicillin-resistant Staphylococcus aureus (MRSA) is a critical health issue that has drawn greater attention to the potential use of immunotherapy. Toll-like receptor 2 (TLR2), a pattern recognition receptor, is an essential component in host innate defense system against S. aureus infection. However, little is known about the innate immune response, specifically TLR2 activation, against MRSA infection. Here, we evaluate the protective effect and the mechanism of MRSA murine pneumonia after pretreatment with Pam3CSK4, a TLR2 agonist. We found that the MRSA-pneumonia mouse model, pretreated with Pam3CSK4, had reduced bacteria and mortality in comparison to control mice. As well, lower protein and mRNA levels of TNF-α, IL-1β and IL-6 were observed in lungs and bronchus of the Pam3CSK4 pretreatment group. Conversely, expression of anti-inflammatory cytokine IL-10, but not TGF-β, increased in Pam3CSK4-pretreated mice. Our additional studies showed that CXCL-2 and CXCL1, which are necessary for neutrophil recruitment, were less evident in the Pam3CSK4-pretreated group compared to control group, whereas the expression of Fcγ receptors (FcγⅠ/Ⅲ) and complement receptors (CR1/3) increased in murine lungs. Furthermore, we found that increased survival and improved bacterial clearance were not a result of higher levels of neutrophil infiltration, but rather a result of enhanced phagocytosis and bactericidal activity of neutrophils in vitro and in vivo as well as increased robust oxidative activity and release of lactoferrin. Our cumulative findings suggest that Pam3CSK4 could be a novel immunotherapeutic candidate against MRSA pneumonia. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e201027d6451b0bb347d5bb3901460feTest http://europepmc.org/articles/PMC4790907Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e201027d6451b0bb347d5bb3901460fe |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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