المؤلفون: Alfonso, Victoria, Maroniche, Guillermo Andrés, Reca, Sol Rita, López, María Gabriela, del Vas, Mariana, Taboga, Oscar Alberto

مصطلحات موضوعية: AcMNPV, ac109, Baculovirus, occlusion, https://purl.org/becyt/ford/1.6Test, https://purl.org/becyt/ford/1Test

الوصف: Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene hasbeen previously characterized as an essential late gene. Our results showed thatbudded virions could be detected in supernatants of infected Sf-9 cells, even whenac109 knockout viruses displayed a single-cell infection phenotype. Moreover, confocalmicroscopy analysis revealed that budded virions can enter the cytoplasm but areunable to enter the cell nucleus. This defect could be repaired by complementingac109 in trans. In addition, polyhedra of normal size could be detected in Sf-9 nucleiinfected with ac109 knockout viruses. However, electron microscopy demonstratedthat these occlusion bodies were empty. Altogether these results indicate that ac109 isrequired for infectivity of both phenotypes of virus. ; Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina ; Fil: Reca, Sol Rita. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: López, María Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ...

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/11336/197050Test; Alfonso, Victoria; Maroniche, Guillermo Andrés; Reca, Sol Rita; López, María Gabriela; del Vas, Mariana; et al.; AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny; Public Library of Science; Plos One; 7; 9; 8-2012; 1-10; CONICET Digital; CONICET

الإتاحة: https://doi.org/10.1371/journal.pone.0046146Test
http://hdl.handle.net/11336/197050Test

المؤلفون: Alfonso, Victoria, Maroniche, Guillermo A., Reca, Sol R., López, María Gabriela, del Vas, Mariana, Taboga, Oscar, Chen Liang

المصدر: PLoS ONE; Sep2012, Vol. 7 Issue 9, Special section p1-10, 10p

مصطلحات موضوعية: ALFALFA looper, NUCLEOPOLYHEDROVIRUSES, CONFOCAL microscopy, VIRION, CYTOPLASM, ELECTRON microscopy

مستخلص: The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of infected Sf-9 cells, even when ac109 knockout viruses displayed a single-cell infection phenotype. Moreover, confocal microscopy analysis revealed that budded virions can enter the cytoplasm but are unable to enter the cell nucleus. This defect could be repaired by complementing ac109 in trans. In addition, polyhedra of normal size could be detected in Sf-9 nuclei infected with ac109 knockout viruses. However, electron microscopy demonstrated that these occlusion bodies were empty. Altogether, these results indicate that ac109 is required for infectivity of both phenotypes of virus. [ABSTRACT FROM AUTHOR]

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