Dendritic Cell Cross-Priming Is Essential for Immune Responses to Listeria monocytogenes
| العنوان: | Dendritic Cell Cross-Priming Is Essential for Immune Responses to Listeria monocytogenes |
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| المؤلفون: | Wilfred A. Jefferies, Kyla D. Omilusik, Genc Basha, Anna T. Reinicke |
| المصدر: | PLoS ONE PLoS ONE, Vol 4, Iss 10, p e7210 (2009) |
| بيانات النشر: | Public Library of Science, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Antigen presentation, Immunology, lcsh:Medicine, Priming (immunology), Bone Marrow Cells, Mice, Transgenic, Biology, CD8-Positive T-Lymphocytes, Listeria infection, Lymphocyte Activation, Microbiology, 03 medical and health sciences, Mice, 0302 clinical medicine, Immune system, Cross-Priming, Antigen, Immunology/Immunity to Infections, medicine, Cytotoxic T cell, Animals, Listeriosis, lcsh:Science, Antigen-presenting cell, 030304 developmental biology, Cell Proliferation, 0303 health sciences, Antigen Presentation, Mice, Inbred C3H, Multidisciplinary, lcsh:R, Dendritic cell, Dendritic Cells, medicine.disease, Listeria monocytogenes, 3. Good health, Mice, Inbred C57BL, Immune System, Immunology/Immune Response, lcsh:Q, 030215 immunology, Research Article |
| الوصف: | Cross-presentation is now recognized as a major mechanism for initiating CD8 T cell responses to virus and tumor antigens in vivo. It provides an elegant mechanism that allows relatively few Dendritic cells (DCs) to initiate primary immune responses while avoiding the consumptive nature of pathogenic infection. CD8 T cells play a major role in anti-bacterial immune responses; however, the contribution of cross-presentation for priming CD8 T cell responses to bacteria, in vivo, is not well established. Listeria monocytogenes (Listeria) is the causative agent of Listeriosis, an opportunistic food-borne bacterial infection that poses a significant public health risk. Here, we employ a transgenic mouse model in which cross-presentation is uniquely inactivated, to investigate cross-priming during primary Listeria infection. We show that cross-priming deficient mice are severely compromised in their ability to generate antigen-specific T cells to stimulate MHC I-restricted CTL responses following Listeria infection. The defect in generation of Listeria-elicited CD8 T cell responses is also apparent in vitro. However, in this setting, the endogenous route of processing Listeria-derived antigens is predominant. This reveals a new experimental dichotomy whereby functional sampling of Listeria-derived antigens in vivo but not in vitro is dependent on cross-presentation of exogenously derived antigen. Thus, under normal physiological circumstances, cross-presentation is demonstrated to play an essential role in priming CD8 T cell responses to bacteria. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6940e271d2964cf83701b9707442556Test http://europepmc.org/articles/PMC2751817Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c6940e271d2964cf83701b9707442556 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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