Evaluation of Microbial Bacterial and Fungal Diversity in Cerebrospinal Fluid Shunt Infection
| العنوان: | Evaluation of Microbial Bacterial and Fungal Diversity in Cerebrospinal Fluid Shunt Infection |
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| المؤلفون: | Samuel R. Browd, Christopher E. Pope, Lucas R. Hoffman, Jeffrey G. Ojemann, Danielle M. Zerr, Jay Riva-Cambrin, Tamara D. Simon, Margaret Rosenfeld, Nicole Mayer-Hamblett |
| المصدر: | PLoS ONE PLoS ONE, Vol 9, Iss 1, p e83229 (2014) |
| بيانات النشر: | Public Library of Science, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Bacterial Diseases, Microbiological culture, Infectious Disease Control, medicine.drug_class, Clinical Research Design, Epidemiology, Nosocomial Infections, Antibiotics, Neurosurgery, lcsh:Medicine, Biology, Pediatrics, Communicable Diseases, Infectious Disease Epidemiology, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Cerebrospinal fluid, Infectious Diseases of the Nervous System, RNA, Ribosomal, 16S, medicine, Humans, Microbiome, lcsh:Science, Staphylococcal Infection, 0303 health sciences, Multidisciplinary, Bacteria, 030306 microbiology, lcsh:R, Fungi, Genetic Variation, Sequence Analysis, DNA, biology.organism_classification, Cerebrospinal Fluid Shunts, 3. Good health, Shunt (medical), Infectious Diseases, Neurology, Observational Studies, Pyrosequencing, Medicine, lcsh:Q, Surgery, Anaerobic bacteria, 030217 neurology & neurosurgery, Research Article, Hydrocephalus |
| الوصف: | Background: Cerebrospinal fluid shunt infection can be recalcitrant. Recurrence is common despite appropriate therapy for the pathogens identified by culture. Improved diagnostic and therapeutic approaches are required, and cultureindependent molecular approaches to cerebrospinal fluid shunt infections have not been described. Objectives: To identify the bacteria and fungi present in cerebrospinal fluid from children with cerebrospinal fluid shunt infection using a high-throughput sequencing approach, and to compare those results to those from negative controls and conventional culture. Methods: This descriptive study included eight children #18 years old undergoing treatment for culture-identified cerebrospinal fluid shunt infection. After routine aerobic culture of each cerebrospinal fluid sample, deoxyribonucleic acid (DNA) extraction was followed by amplification of the bacterial 16S rRNA gene and the fungal ITS DNA region tag-encoded FLX-Titanium amplicon pyrosequencing and microbial phylogenetic analysis. Results: The microbiota analyses for the initial cerebrospinal fluid samples from all eight infections identified a variety of bacteria and fungi, many of which did not grow in conventional culture. Detection by conventional culture did not predict the relative abundance of an organism by pyrosequencing, but in all cases, at least one bacterial taxon was detected by both conventional culture and pyrosequencing. Individual bacterial species fluctuated in relative abundance but remained above the limits of detection during infection treatment. Conclusions: Numerous bacterial and fungal organisms were detected in these cerebrospinal fluid shunt infections, even during and after treatment, indicating diverse and recalcitrant shunt microbiota. In evaluating cerebrospinal fluid shunt infection, fungal and anaerobic bacterial cultures should be considered in addition to aerobic bacterial cultures, and cultureindependent approaches offer a promising alternative diagnostic approach. More effective treatment of cerebrospinal fluid shunt infections is needed to reduce unacceptably high rates of reinfection, and this work suggests that one effective strategy may be reduction of the diverse microbiota present in infection. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97022ad6b8ba59c8ff9104fd5148c323Test http://europepmc.org/articles/PMC3885436Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....97022ad6b8ba59c8ff9104fd5148c323 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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