Serum miR-33a is associated with steatosis and inflammation in patients with non-alcoholic fatty liver disease after liver transplantation
| العنوان: | Serum miR-33a is associated with steatosis and inflammation in patients with non-alcoholic fatty liver disease after liver transplantation |
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| المؤلفون: | Helena Dankova, Eva Sticova, Ondrej Seda, Pavel Trunecka, Denisa Erhartová, Monika Cahova, Marie Heczkova, Irena Míková, Julius Spicak |
| المصدر: | PLoS ONE PLoS ONE, Vol 14, Iss 11, p e0224820 (2019) |
| بيانات النشر: | Public Library of Science, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Steatosis, medicine.medical_treatment, Biopsy, Liver transplantation, Pathology and Laboratory Medicine, Gastroenterology, Biochemistry, Cytopathology, 0302 clinical medicine, Fibrosis, Non-alcoholic Fatty Liver Disease, Medicine and Health Sciences, Immune Response, Multidisciplinary, medicine.diagnostic_test, Liver Diseases, Fatty liver, Middle Aged, Lipids, Nucleic acids, Liver biopsy, Medicine, 030211 gastroenterology & hepatology, Female, Research Article, Adult, medicine.medical_specialty, Science, Immunology, Surgical and Invasive Medical Procedures, Gastroenterology and Hepatology, 03 medical and health sciences, Digestive System Procedures, Insulin resistance, Signs and Symptoms, Diagnostic Medicine, Internal medicine, medicine, Genetics, Humans, Circulating MicroRNA, Non-coding RNA, Aged, Inflammation, Transplantation, Natural antisense transcripts, business.industry, Biology and Life Sciences, Organ Transplantation, medicine.disease, Lipid Metabolism, Liver Transplantation, Gene regulation, Fatty Liver, MicroRNAs, 030104 developmental biology, Metabolism, ROC Curve, Anatomical Pathology, RNA, Gene expression, Steatohepatitis, business, Dyslipidemia, Biomarkers, Developmental Biology |
| الوصف: | Background & aims MiR-33a has emerged as a critical regulator of lipid homeostasis in the liver. Genetic deficiency of miR-33a aggravates liver steatosis in a preclinical model of non-alcoholic fatty liver disease (NAFLD), and relative expression of miR-33a is increased in the livers of patients with non-alcoholic steatohepatitis (NASH). It was unknown whether miR-33a is detectable in the serum of patients with NAFLD. We sought to determine whether circulating miR-33a is associated with histological hepatic steatosis, inflammation, ballooning or fibrosis, and whether it could be used as a serum marker in patients with NAFLD/NASH. Methods We analysed circulating miR-33a using quantitative PCR in 116 liver transplant recipients who underwent post-transplant protocol liver biopsy. Regression analysis was used to determine association of serum miR-33a with hepatic steatosis, inflammation, ballooning and fibrosis in liver biopsy. Results Liver graft steatosis and inflammation, but not ballooning or fibrosis, were significantly associated with serum miR-33a, dyslipidemia and insulin resistance markers on univariate analysis. Multivariate analysis showed that steatosis was independently associated with serum miR-33a, ALT, glycaemia and waist circumference, whereas inflammation was independently associated with miR-33a, HbA1 and serum triglyceride levels. Receiver operating characteristic analysis showed that exclusion of serum miR-33a from multivariate analysis resulted in non-significant reduction of prediction model accuracy of liver steatosis or inflammation. Conclusions Our data indicate that circulating miR-33a is an independent predictor of liver steatosis and inflammation in patients after liver transplantation. Although statistically significant, its contribution to the accuracy of prediction model employing readily available clinical and biochemical variables was limited in our cohort. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63b6c29ebac7cbf867463a925d887f8eTest http://europepmc.org/articles/PMC6839850Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....63b6c29ebac7cbf867463a925d887f8e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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