Haspin kinase modulates nuclear architecture and Polycomb-dependent gene silencing
| العنوان: | Haspin kinase modulates nuclear architecture and Polycomb-dependent gene silencing |
|---|---|
| المؤلفون: | Maria A. Rodríguez-Sánchez, M. Lluïsa Espinás, Ujué Fresán, Victor G. Corces, Oscar Reina |
| المساهمون: | Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Consejo Superior de Investigaciones Científicas (España) |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname PLoS Genetics, Vol 16, Iss 8, p e1008962 (2020) PLoS Genetics |
| بيانات النشر: | Public Library of Science, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Threonine, Cancer Research, Life Cycles, Euchromatin, Chromosomal Proteins, Non-Histone, Gene Expression, Polycomb-Group Proteins, Cell Cycle Proteins, QH426-470, Salivary Glands, Histones, 0302 clinical medicine, Larvae, Heterochromatin, Chromosome Segregation, Invertebrate Genomics, Medicine and Health Sciences, Drosophila Proteins, Cell Cycle and Cell Division, Heterochromatin organization, Phosphorylation, Materials, Genetics (clinical), 0303 health sciences, Chromosome Biology, Drosophila Melanogaster, Eukaryota, Animal Models, Genomics, Chromatin, Cell biology, Establishment of sister chromatid cohesion, Insects, Experimental Organism Systems, Cell Processes, Physical Sciences, Epigenetics, Drosophila, Anatomy, Homeotic gene, Research Article, Cohesin complex, Arthropoda, Materials Science, Centromere, Mitosis, Biology, Protein Serine-Threonine Kinases, Research and Analysis Methods, 03 medical and health sciences, Model Organisms, Exocrine Glands, Genetics, Animals, Gene Silencing, Molecular Biology, Interphase, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Organisms, Biology and Life Sciences, Cell Biology, Insulators, Invertebrates, Animal Genomics, Animal Studies, Zoology, Entomology, Digestive System, Sister Chromatid Exchange, 030217 neurology & neurosurgery, Developmental Biology |
| الوصف: | © 2020 Fresán et al. Haspin, a highly conserved kinase in eukaryotes, has been shown to be responsible for phosphorylation of histone H3 at threonine 3 (H3T3ph) during mitosis, in mammals and yeast. Here we report that haspin is the kinase that phosphorylates H3T3 in Drosophila melanogaster and it is involved in sister chromatid cohesion during mitosis. Our data reveal that haspin also phosphorylates H3T3 in interphase. H3T3ph localizes in broad silenced domains at heterochromatin and lamin-enriched euchromatic regions. Loss of haspin compromises insulator activity in enhancer-blocking assays and triggers a decrease in nuclear size that is accompanied by changes in nuclear envelope morphology. We show that haspin is a suppressor of position-effect variegation involved in heterochromatin organization. Our results also demonstrate that haspin is necessary for pairing-sensitive silencing and it is required for robust Polycomb-dependent homeotic gene silencing. Haspin associates with the cohesin complex in interphase, mediates Pds5 binding to chromatin and cooperates with Pds5-cohesin to modify Polycomb-dependent homeotic transformations. Therefore, this study uncovers an unanticipated role for haspin kinase in genome organization of interphase cells and demonstrates that haspin is required for homeotic gene regulation. This work was supported by the Spanish Ministerio de Economia y Competitividad (BFU2013-48712-P) and the Generalitat de Catalunya (SGR2017-475). U. F. and M. R-S. acknowledge receipt of doctoral fellowships from Consejo Superior de Investigaciones Cientificas and Ministerio de Economia y Competitividad respectively. |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d61c87a4724ea70e9df48832d456386dTest http://hdl.handle.net/10261/223087Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d61c87a4724ea70e9df48832d456386d |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |