The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine’s Induced Resistance against Plasmopara viticola
| العنوان: | The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine’s Induced Resistance against Plasmopara viticola |
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| المؤلفون: | Mark, Gijzen, Adrien, Gauthier, Sophie, Trouvelot, Jani, Kelloniemi, Patrick, Frettinger, David, Wendehenne, Xavier, Daire, Jean Marie Joubert, Ferrarini, Alberto, Delledonne, Massimo, Victor, Flors, Benoit, Poinssot |
| المساهمون: | Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoires Goëmar S.A.S., Dipartimento di Biotecnologie, University of Verona (UNIVR), Universitat Jaume I |
| المصدر: | Repositori Universitat Jaume I Universitat Jaume I Plos One 2 (9), . (2014) PLoS ONE PLoS ONE, Public Library of Science, 2014, 9 (2), ⟨10.1371/journal.pone.0088145⟩ PLoS ONE, Vol 9, Iss 2, p e88145 (2014) |
| بيانات النشر: | Public Library of Science, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | 0106 biological sciences, [SDV]Life Sciences [q-bio], lcsh:Medicine, laminarine sulfatée, Plant disease resistance, 01 natural sciences, Microbiology, Transcriptome, Plasmopara viticola, 03 medical and health sciences, Laminarin, chemistry.chemical_compound, vitis vinifera, Botany, transcriptome du stress, défenses SA- et ROS- dépendants, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, amorçage, résistance, grapevine, lcsh:Science, 030304 developmental biology, 2. Zero hunger, chemistry.chemical_classification, 0303 health sciences, Reactive oxygen species, Multidisciplinary, biology, Phytoalexin, lcsh:R, biology.organism_classification, microarray, Respiratory burst, Elicitor, chemistry, [SDE]Environmental Sciences, lcsh:Q, 010606 plant biology & botany |
| الوصف: | Grapevine (Vitis vinifera) is susceptible to many pathogens which cause significant losses to viticulture worldwide. Chemical control is available, but agro-ecological concerns have raised interest in alternative methods, especially in triggering plant immunity by elicitor treatments. The b-glucan laminarin (Lam) and its sulfated derivative (PS3) have been previously demonstrated to induce resistance in grapevine against downy mildew (Plasmopara viticola). However, if Lam elicits classical grapevine defenses such as oxidative burst, pathogenesis-related (PR)-proteins and phytoalexin production, PS3 triggered grapevine resistance via a poorly understood priming phenomenon. The aim of this study was to identify the molecular mechanisms of the PS3-induced resistance. For this purpose we studied i) the signaling events and transcriptome reprogramming triggered by PS3 treatment on uninfected grapevine, ii) grapevine immune responses primed by PS3 during P. viticola infection. Our results showed that i) PS3 was unable to elicit reactive oxygen species (ROS) production, cytosolic Ca2+ concentration variations, mitogen-activated protein kinase (MAPK) activation but triggered a long lasting plasma membrane depolarization in grapevine cells, ii) PS3 and Lam shared a common stress-responsive transcriptome profile that partly overlapped the salicylate- (SA) and jasmonate-(JA)-dependent ones. After P. viticola inoculation, PS3 specifically primed the SA- and ROS-dependent defense pathways leading to grapevine induced resistance against this biotroph. Interestingly pharmacological approaches suggested that the plasma membrane depolarization and the downstream ROS production are key events of the PS3-induced resistance |
| وصف الملف: | application/pdf |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57abe2ade5830b9c0d9b91cfce35e307Test http://hdl.handle.net/10234/123736Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....57abe2ade5830b9c0d9b91cfce35e307 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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