Azoospermia in mice with targeted disruption of the Brek/Lmtk2 (brain-enriched kinase/lemur tyrosine kinase 2) gene
| العنوان: | Azoospermia in mice with targeted disruption of the Brek/Lmtk2 (brain-enriched kinase/lemur tyrosine kinase 2) gene |
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| المؤلفون: | Chizuru Ito, Yoshiro Toyama, Mamiko Maekawa, Kazumasa Yokoyama, Takahisa Nakamura, Tadashi Yamamoto, Nobuaki Yoshida, Takanobu Nakazawa, Kiyotaka Toshimori, Seiji Kawa, Tohru Tezuka |
| المصدر: | Proceedings of the National Academy of Sciences. 103:19344-19349 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Male, endocrine system, Somatic cell, Immunoblotting, Protein Serine-Threonine Kinases, Biology, LMTK2, Mice, Microscopy, Electron, Transmission, Testis, medicine, Animals, Spermatogenesis, Azoospermia, Mice, Knockout, Multidisciplinary, Protein-Serine-Threonine Kinases, Reverse Transcriptase Polymerase Chain Reaction, Kinase, Biological Sciences, medicine.disease, Immunohistochemistry, Cell biology, Fertility, Tyrosine kinase 2, Cancer research |
| الوصف: | Brek/Lmtk2 (brain-enriched kinase/lemur tyrosine kinase 2) is a member of the Aatyk family of kinases that comprises Aatyk1, Brek/Lmtk2/Aatyk2, and Aatyk3. Although several potential roles have been proposed for Brek and other Aatyk family members, the physiological functions of these kinases remain unclear. Here, we report that Brek −/− male mice are infertile, with azoospermia. Detailed histological analysis revealed that Brek −/− germ cells differentiated normally until the round-spermatid stage, but failed to undergo the normal change in morphology to become elongated spermatids. Testicular somatic cells appeared normal in these mice. Expression of Brek in testis was restricted to the germ cells, suggesting that the maturations of germ cells in Brek −/− mice are affected in a cell-autonomous manner. On the basis of these findings, we concluded that Brek is essential for a late stage of spermatogenesis. Further clarification of the mechanism by which Brek regulates spermatogenesis may help identify new targets for reproductive contraceptives and treatments against infertility. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de18c35e793925c4b50c02c002c5dfd1Test https://doi.org/10.1073/pnas.0603603103Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....de18c35e793925c4b50c02c002c5dfd1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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