Short-chain diamines are the physiological substrates of PACE family efflux pumps
| العنوان: | Short-chain diamines are the physiological substrates of PACE family efflux pumps |
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| المؤلفون: | Liping Li, Qi Liu, Jacob R. Edgerton, Leila Fahmy, Irshad Ahmad, Ian T. Paulsen, Karla A. Mettrick, Karl A. Hassan, Varsha Naidu, David Sharples, Peter J. F. Henderson, Scott M. Jackson |
| المصدر: | Proceedings of the National Academy of Sciences. 116:18015-18020 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Acinetobacter baumannii, Cadaverine transport, Diamines, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, Drug Resistance, Bacterial, cardiovascular diseases, 030304 developmental biology, 0303 health sciences, Cadaverine, Multidisciplinary, biology, 030306 microbiology, Chemistry, Chlorhexidine, Membrane Transport Proteins, Biological Sciences, Membrane transport, biology.organism_classification, Anti-Bacterial Agents, 3. Good health, Transport protein, Putrescine, Efflux, Bacteria |
| الوصف: | Acinetobacter baumannii has rapidly emerged as a major cause of gram-negative hospital infections worldwide. A. baumannii encodes for the transport protein AceI, which confers resistance to chlorhexidine, a widely used antiseptic. AceI is also the prototype for the recently discovered proteobacterial antimicrobial compound efflux (PACE) family of transport proteins that confer resistance to a range of antibiotics and antiseptics in many gram-negative bacteria, including pathogens. The gene encoding AceI is conserved in the core genome of A. baumannii, suggesting that it has an important primordial function. This is incongruous with the sole characterized substrate of AceI, chlorhexidine, an entirely synthetic biocide produced only during the last century. Here we investigated a potential primordial function of AceI and other members of the PACE family in the transport of naturally occurring polyamines. Polyamines are abundant in living cells, where they have physiologically important functions and play multifaceted roles in bacterial infection. Gene expression studies revealed that the aceI gene is induced in A. baumannii by the short-chain diamines cadaverine and putrescine. Membrane transport experiments conducted in whole cells of A. baumannii and Escherichia coli and also in proteoliposomes showed that AceI mediates the efflux of these short-chain diamines when energized by an electrochemical gradient. Assays conducted using 8 additional diverse PACE family proteins identified 3 that also catalyze cadaverine transport. Taken together, these results demonstrate that short-chain diamines are common substrates for the PACE family of transport proteins, adding to their broad significance as a novel family of efflux pumps. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfd2c9646691bd27610999112f25ba1cTest https://doi.org/10.1073/pnas.1901591116Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dfd2c9646691bd27610999112f25ba1c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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