Genome-wide suppression of aberrant mRNA-like noncoding RNAs by NMD in Arabidopsis
| العنوان: | Genome-wide suppression of aberrant mRNA-like noncoding RNAs by NMD in Arabidopsis |
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| المؤلفون: | Motoaki Seki, Tetsuro Toyoda, Kazuo Shinozaki, Yukio Kurihara, Taeko Morosawa, Eli Kaminuma, Yoshiki Mochizuki, Akihiro Matsui, Kousuke Hanada, Junko Ishida, Maho Tanaka, Makiko Kawashima, Akihiro Matsushima |
| المصدر: | Proceedings of the National Academy of Sciences. 106:2453-2458 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | RNA, Untranslated, Arabidopsis, Genes, Plant, Models, Biological, Exon, Gene Expression Regulation, Plant, Transcription (biology), Gene expression, RNA, Messenger, Cycloheximide, Gene, Protein Synthesis Inhibitors, Genetics, Multidisciplinary, Tiling array, Models, Genetic, biology, Arabidopsis Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA, Exons, Biological Sciences, biology.organism_classification, Antisense RNA, Mutation, Genome, Plant |
| الوصف: | The nonsense-mediated mRNA decay (NMD) pathway is a well-known eukaryotic surveillance mechanism that eliminates aberrant mRNAs that contain a premature termination codon (PTC). The UP-Frameshift (UPF) proteins, UPF1, UPF2, and UPF3, are essential for normal NMD function. Several NMD substrates have been identified, but detailed information on NMD substrates is lacking. Here, we noticed that, in Arabidopsis , most of the mRNA-like nonprotein-coding RNAs (ncRNAs) have the features of an NMD substrate. We examined the expression profiles of 2 Arabidopsis mutants, upf1-1 and upf3-1 , using a whole-genome tiling array. The results showed that expression of not only protein-coding transcripts but also many mRNA-like ncRNAs (mlncRNAs), including natural antisense transcript RNAs (nat-RNAs) transcribed from the opposite strands of the coding strands, were up-regulated in both mutants. The percentage of the up-regulated mlncRNAs to all expressed mlncRNAs was much higher than that of the up-regulated protein-coding transcripts to all expressed protein- coding transcripts. This finding demonstrates that one of the most important roles of NMD is the genome-wide suppression of the aberrant mlncRNAs including nat-RNAs. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cd63fed3fb0dd32b3b9433f6fd004aaTest https://doi.org/10.1073/pnas.0808902106Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1cd63fed3fb0dd32b3b9433f6fd004aa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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