Secretory IgA-mediated protection against V. cholerae and heat-labile enterotoxin-producing enterotoxigenic Escherichia coli by rice-based vaccine
| العنوان: | Secretory IgA-mediated protection against V. cholerae and heat-labile enterotoxin-producing enterotoxigenic Escherichia coli by rice-based vaccine |
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| المؤلفون: | Takeshi Honda, Toshio Kodama, Tomonori Nochi, Shiho Kurokawa, Fumio Takaiwa, Yuko Takahashi, Masanobu Nanno, Yoshikazu Yuki, Mio Mejima, Daisuke Tokuhara, Hiroshi Kiyono, Ushio Nakanishi |
| المصدر: | Proceedings of the National Academy of Sciences. 107:8794-8799 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Diarrhea, Cholera Toxin, Hot Temperature, Cross Protection, Administration, Oral, Heat-labile enterotoxin, Biology, medicine.disease_cause, complex mixtures, Microbiology, Enterotoxins, Mice, Enterotoxigenic Escherichia coli, medicine, Animals, Receptors, Immunologic, Vibrio cholerae, Mice, Knockout, Mice, Inbred BALB C, Multidisciplinary, Escherichia coli Vaccines, Vaccination, Cholera toxin, Immunity, Cholera Vaccines, Oryza, Biological Sciences, medicine.disease, Cholera, Virology, embryonic structures, Immunoglobulin A, Secretory, Female, medicine.symptom, Cholera vaccine |
| الوصف: | Cholera and enterotoxigenic Escherichia coli (ETEC) are among the most common causes of acute infantile gastroenteritis globally. We previously developed a rice-based vaccine that expressed cholera toxin B subunit (MucoRice-CTB) and had the advantages of being cold chain–free and providing protection against cholera toxin (CT)–induced diarrhea. To advance the development of MucoRice-CTB for human clinical application, we investigated whether the CTB-specific secretory IgA (SIgA) induced by MucoRice-CTB gives longstanding protection against diarrhea induced by Vibrio cholerae and heat-labile enterotoxin (LT)–producing ETEC (LT-ETEC) in mice. Oral immunization with MucoRice-CTB stored at room temperature for more than 3 y provided effective SIgA-mediated protection against CT- or LT-induced diarrhea, but the protection was impaired in polymeric Ig receptor–deficient mice lacking SIgA. The vaccine gave longstanding protection against CT- or LT-induced diarrhea (for ≥6 months after primary immunization), and a single booster immunization extended the duration of protective immunity by at least 4 months. Furthermore, MucoRice-CTB vaccination prevented diarrhea in the event of V. cholerae and LT-ETEC challenges. Thus, MucoRice-CTB is an effective long-term cold chain–free oral vaccine that induces CTB-specific SIgA-mediated longstanding protection against V. cholerae – or LT-ETEC–induced diarrhea. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15cc36533b91e2b59dd267aa3601fbebTest https://doi.org/10.1073/pnas.0914121107Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....15cc36533b91e2b59dd267aa3601fbeb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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