Endothelial expression of hypoxia-inducible factor 1 protects the murine heart and aorta from pressure overload by suppression of TGF-β signaling
| العنوان: | Endothelial expression of hypoxia-inducible factor 1 protects the murine heart and aorta from pressure overload by suppression of TGF-β signaling |
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| المؤلفون: | Jasper Chen, Norimichi Koitabashi, Dongmei Xing, Djahida Bedja, Shaoping Chen, John W. Harmon, Rosanne Rouf, Harry C. Dietz, Hong Wei, Charles Steenbergen, Gregg L. Semenza, Kathleen L. Gabrielson, David A. Kass, Karen Fox-Talbot |
| المصدر: | Proceedings of the National Academy of Sciences. 109 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | medicine.medical_specialty, Endothelium, Cardiac fibrosis, Smad Proteins, Mice, Transforming Growth Factor beta, medicine.artery, Internal medicine, Pressure, medicine, Animals, Extracellular Signal-Regulated MAP Kinases, Aorta, Mice, Knockout, Pressure overload, Multidisciplinary, biology, business.industry, Heart, Transforming growth factor beta, Hypoxia-Inducible Factor 1, alpha Subunit, medicine.disease, medicine.anatomical_structure, Endocrinology, PNAS Plus, Heart failure, cardiovascular system, biology.protein, Endothelium, Vascular, Signal transduction, business, Homeostasis, Signal Transduction |
| الوصف: | Chronic systemic hypertension causes cardiac pressure overload leading to increased myocardial O 2 consumption. Hypoxia-inducible factor 1 (HIF-1) is a master regulator of O 2 homeostasis. Mouse embryos lacking expression of the O 2 -regulated HIF-1α subunit die at midgestation with severe cardiac malformations and vascular regression. Here we report that Hif1a f/f ;Tie2-Cre conditional knockout mice, which lack HIF-1α expression only in Tie2 + lineage cells, develop normally, but when subjected to pressure overload induced by transaortic constriction (TAC), they manifest rapid cardiac decompensation, which is accompanied by excess cardiac fibrosis and myocardial hypertrophy, decreased myocardial capillary density, increased myocardial hypoxia and apoptosis, and increased TGF-β signaling through both canonical and noncanonical pathways that activate SMAD2/3 and ERK1/2, respectively, within endothelial cells of cardiac blood vessels. TAC also induces dilatation of the proximal aorta through enhanced TGF-β signaling in Hif1a f/f ;Tie2-Cre mice. Inhibition of TGF-β signaling by treatment with neutralizing antibody or pharmacologic inhibition of MEK–ERK signaling prevented TAC-induced contractile dysfunction and pathological remodeling. Thus, HIF-1 plays a critical protective role in the adaptation of the heart and aorta to pressure overload by negatively regulating TGF-β signaling in endothelial cells. Treatment of wild-type mice with digoxin, which inhibits HIF-1α synthesis, resulted in rapid cardiac failure after TAC. Although digoxin has been used for decades as an inotropic agent to treat heart failure, it does not improve survival, suggesting that the countertherapeutic effects of digoxin observed in the TAC mouse model may have clinical relevance. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7894214c8b324c569d5f3fc131e733cTest https://doi.org/10.1073/pnas.1202081109Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c7894214c8b324c569d5f3fc131e733c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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