Electron tomography reveals the fibril structure and lipid interactions in amyloid deposits
| العنوان: | Electron tomography reveals the fibril structure and lipid interactions in amyloid deposits |
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| المؤلفون: | Uwe Horn, Julia Linder, Marius Kollmer, Volker Schmidt, Falk Liberta, Tatiana Syrovets, Marcus Fändrich, Thomas Simmet, Katrin Meinhardt, Per Westermark, Gunilla T. Westermark, Matthias Schmidt, Paul Walther, Liesa Heinrich, Christian Haupt, Melanie Wulff, Lisa Handl, Cornelia Loos |
| المصدر: | Proceedings of the National Academy of Sciences. 113:5604-5609 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Amyloid, Electron Microscope Tomography, Lipid Bilayers, Nanotechnology, macromolecular substances, Fibril, Mice, 03 medical and health sciences, medicine, Animals, Serum amyloid A, Lipid bilayer, Cells, Cultured, Serum Amyloid A Protein, Multidisciplinary, Chemistry, Amyloidosis, Biological Sciences, medicine.disease, Lipids, 030104 developmental biology, Electron tomography, Biophysics, Female |
| الوصف: | Electron tomography is an increasingly powerful method to study the detailed architecture of macromolecular complexes or cellular structures. Applied to amyloid deposits formed in a cell culture model of systemic amyloid A amyloidosis, we could determine the structural morphology of the fibrils directly in the deposit. The deposited fibrils are arranged in different networks, and depending on the relative fibril orientation, we can distinguish between fibril meshworks, fibril bundles, and amyloid stars. These networks are frequently infiltrated by vesicular lipid inclusions that may originate from the death of the amyloid-forming cells. Our data support the role of nonfibril components for constructing fibril deposits and provide structural views of different types of lipid-fibril interactions. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee701dcd05797d4e35f4d131634f06d1Test https://doi.org/10.1073/pnas.1523496113Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ee701dcd05797d4e35f4d131634f06d1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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