Release of amyloid beta-protein precursor derivatives by electrical depolarization of rat hippocampal slices
| العنوان: | Release of amyloid beta-protein precursor derivatives by electrical depolarization of rat hippocampal slices |
|---|---|
| المؤلفون: | Roger Nitsch, Steven A. Farber, John H. Growdon, Richard J. Wurtman |
| المصدر: | Proceedings of the National Academy of Sciences. 90:5191-5193 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | Male, Amyloid beta, Immunoblotting, Tetrodotoxin, In Vitro Techniques, Hippocampal formation, Neurotransmission, Hippocampus, Rats, Sprague-Dawley, Amyloid beta-Protein Precursor, chemistry.chemical_compound, mental disorders, Amyloid precursor protein, medicine, Animals, Neurotransmitter, Amyloid beta-Peptides, Multidisciplinary, L-Lactate Dehydrogenase, biology, Depolarization, Acetylcholine, Electric Stimulation, Rats, Perfusion, chemistry, Biochemistry, biology.protein, Biophysics, Research Article, medicine.drug |
| الوصف: | Proteolytic processing of the beta-amyloid protein precursor (APP) is regulated by cell-surface receptors. To determine whether neurotransmitter release in response to neuronal activation regulates APP processing in brain, we electrically depolarized superfused rat hippocampal slices and measured soluble APP derivatives released into the superfusate. Electrical depolarization caused a rapid increase in the release of both neurotransmitters and amino-terminal APP cleavage products. These derivatives lacked the APP carboxyl terminus and were similar to those found in both cell culture media and human cerebrospinal fluid. Superfusate proteins including lactate dehydrogenase were not changed by electrical depolarization. The release of amino-terminal APP derivatives increased with increasing stimulation frequencies from 0 to 30 Hz. The increased release was inhibited by the sodium-channel antagonist tetrodotoxin, suggesting that action-potential formation mediates the release of large amino-terminal APP derivatives. These results suggest that neuronal activity regulates APP processing in the mammalian brain. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46cbd92fae9eb3f8ce689b8420d6d2a3Test https://doi.org/10.1073/pnas.90.11.5191Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....46cbd92fae9eb3f8ce689b8420d6d2a3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
|---|