Bacillus subtilis chromosome organization oscillates between two distinct patterns
| العنوان: | Bacillus subtilis chromosome organization oscillates between two distinct patterns |
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| المؤلفون: | Paula Montero Llopis, Xindan Wang, David Z. Rudner |
| المصدر: | Proceedings of the National Academy of Sciences. 111:12877-12882 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Chromosome segregation, Genetics, Condensin complex, Multidisciplinary, biology, Caulobacter crescentus, Circular bacterial chromosome, DNA replication, Chromosome, Bacillus subtilis, Ploidy, biology.organism_classification |
| الوصف: | Bacterial chromosomes have been found to possess one of two distinct patterns of spatial organization. In the first, called “ori-ter” and exemplified by Caulobacter crescentus, the chromosome arms lie side-by-side, with the replication origin and terminus at opposite cell poles. In the second, observed in slow-growing Escherichia coli (“left-ori-right”), the two chromosome arms reside in separate cell halves, on either side of a centrally located origin. These two patterns, rotated 90° relative to each other, appear to result from different segregation mechanisms. Here, we show that the Bacillus subtilis chromosome alternates between them. For most of the cell cycle, newly replicated origins are maintained at opposite poles with chromosome arms adjacent to each other, in an ori-ter configuration. Shortly after replication initiation, the duplicated origins move as a unit to midcell and the two unreplicated arms resolve into opposite cell halves, generating a left-ori-right pattern. The origins are then actively segregated toward opposite poles, resetting the cycle. Our data suggest that the condensin complex and the parABS partitioning system are the principal driving forces underlying this oscillatory cycle. We propose that the distinct organization patterns observed for bacterial chromosomes reflect a common organization–segregation mechanism, and that simple modifications to it underlie the unique patterns observed in different species. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::9c17f0daf3061e3094106b05be9a3ebfTest https://doi.org/10.1073/pnas.1407461111Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........9c17f0daf3061e3094106b05be9a3ebf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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