The effect of 4,4′-diisothiocyanato-stilbene-2,2′-disulfonate on CO 2 permeability of the red blood cell membrane
| العنوان: | The effect of 4,4′-diisothiocyanato-stilbene-2,2′-disulfonate on CO 2 permeability of the red blood cell membrane |
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| المؤلفون: | Gerolf Gros, Y. Ono, R. E. Forster, Michael B. Wunder, L. Lin |
| المصدر: | Proceedings of the National Academy of Sciences. 95:15815-15820 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Adult, Cell Membrane Permeability, Erythrocytes, Time Factors, 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid, In Vitro Techniques, Oxygen Isotopes, Models, Biological, chemistry.chemical_compound, Anion Exchange Protein 1, Erythrocyte, medicine, Humans, Lipid bilayer, Carbonic Anhydrases, Carbon Isotopes, Multidisciplinary, Chromatography, biology, Membrane transport protein, Bilayer, Erythrocyte Membrane, Biological Sciences, Carbon Dioxide, Transport protein, Bicarbonates, Kinetics, Red blood cell, Membrane, medicine.anatomical_structure, Models, Chemical, chemistry, DIDS, biology.protein, Biophysics, Intracellular |
| الوصف: | It has long been assumed that the red cell membrane is highly permeable to gases because the molecules of gases are small, uncharged, and soluble in lipids, such as those of a bilayer. The disappearance of 12 C 18 O 16 O from a red cell suspension as the 18 O exchanges between labeled CO 2 + HCO 3 − and unlabeled HOH provides a measure of the carbonic anhydrase (CA) activity (acceleration, or A ) inside the cell and of the membrane self-exchange permeability to HCO 3 − ( P m,HCO − 3 ). To test this technique, we added sufficient 4,4′-diisothiocyanato-stilbene-2,2′-disulfonate (DIDS) to inhibit all the HCO 3 − /Cl − transport protein (Band III or capnophorin) in a red cell suspension. We found that DIDS reduced P m,HCO − 3 as expected, but also appeared to reduce intracellular A , although separate experiments showed it has no effect on CA activity in homogenous solution. A decrease in P m,CO 2 would explain this finding. With a more advanced computational model, which solves for CA activity and membrane permeabilities to both CO 2 and HCO 3 − , we found that DIDS inhibited both P m,HCO − 3 and P m,CO 2 , whereas intracellular CA activity remained unchanged. The mechanism by which DIDS reduces CO 2 permeability may not be through an action on the lipid bilayer itself, but rather on a membrane transport protein, implying that this is a normal route for at least part of red cell CO 2 exchange. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf2467b42e0a5926eae1a172baa61089Test https://doi.org/10.1073/pnas.95.26.15815Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bf2467b42e0a5926eae1a172baa61089 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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