High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae)
| العنوان: | High Bacterial Agglutination Activity in a Single-CRD C-Type Lectin from Spodoptera exigua (Lepidoptera: Noctuidae) |
|---|---|
| المؤلفون: | Leila Gasmi, Juan Ferré, Salvador Herrero |
| المصدر: | Biosensors Biosensors, Vol 7, Iss 1, p 12 (2017) Biosensors; Volume 7; Issue 1; Pages: 12 |
| بيانات النشر: | Preprints, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, agglutination, lcsh:Biotechnology, Clinical Biochemistry, Microbial Sensitivity Tests, Spodoptera, C-type lectin, CRD, bacterial detection, E. coli, Article, Microbiology, law.invention, Lepidoptera genitalia, 03 medical and health sciences, 0302 clinical medicine, law, lcsh:TP248.13-248.65, Agglutination Tests, Exigua, Escherichia coli, Animals, Lectins, C-Type, Amino Acid Sequence, Conserved Sequence, Phylogeny, biology, General Medicine, biology.organism_classification, Immunity, Innate, Agglutination (biology), 030104 developmental biology, Recombinant DNA, Noctuidae, Insect Proteins, Protein concentration, 030215 immunology, biotechnology |
| الوصف: | Lectins are carbohydrate-interacting proteins that play a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensors for capturing and identifying bacterial species. In this work, three C-type lectins from the Lepidoptera Spodoptera exigua were produced as recombinant proteins and their bacterial agglutination properties were characterized. The lowest protein concentration producing bacterial agglutination against a panel of different Gram+ and Gram− as well as their carbohydrate binding specificities was determined for the three lectins. One of these lectins, BLL2, was able to agglutinate cells from a broad range of bacterial species at an extremely low concentration, becoming a very interesting protein to be used as a biosensor or for other biotechnological applications involving bacterial capture. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aef6a312eea05ba177b7de0056f85ca6Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....aef6a312eea05ba177b7de0056f85ca6 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |