Silencing microRNA-27a inhibits proliferation and invasion of human osteosarcoma cells through the SFRP1-dependent Wnt/β-catenin signaling pathway
| العنوان: | Silencing microRNA-27a inhibits proliferation and invasion of human osteosarcoma cells through the SFRP1-dependent Wnt/β-catenin signaling pathway |
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| المؤلفون: | Chen Xue, Li Junfeng, Lina Zhang, Yu Mu, Yuanyuan Shi, Si Chen |
| المصدر: | Bioscience Reports |
| بيانات النشر: | Portland Press Ltd., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Wnt/β-catenin signaling pathway, Proliferation, MicroRNA-27a, Biochemistry, Metastasis, 0302 clinical medicine, Invasion, Child, Wnt Signaling Pathway, Research Articles, beta Catenin, Osteosarcoma, Wnt signaling pathway, Middle Aged, Gene Expression Regulation, Neoplastic, 030220 oncology & carcinogenesis, SFRP1, Disease Progression, Intercellular Signaling Peptides and Proteins, Female, Signal transduction, Research Article, Adult, Adolescent, Biophysics, Bone Neoplasms, Biology, Young Adult, 03 medical and health sciences, Secreted frizzled-related protein 1, Cell Line, Tumor, Microrna 27a, medicine, Humans, Gene silencing, Neoplasm Invasiveness, Gene Silencing, Molecular Biology, Gene, Cell Proliferation, Membrane Proteins, Cell Biology, medicine.disease, MicroRNAs, 030104 developmental biology, Cancer research |
| الوصف: | Osteosarcoma is the most common malignant tumor of bone with a high potential for metastasis. Importantly, microRNA-27a (miR-27a) is involved in the progression of osteosarcoma. The present study aims to discuss the effects of miR-27a and its target gene secreted frizzled related protein 1 (SFRP1) on proliferation and invasion of human osteosarcoma cells via Wnt/β-catenin signaling pathway. The expression of miR-27a and SFRP1 in osteosarcoma tissues and cells was detected, followed by identification of their relations. Subsequently, miR-27a mimic, miR-27a inhibitor, or siRNA against SFRP1 were introduced into cells (HOS and U2OS) to investigate their role in cell proliferation and invasion. The expression of Wnt/β-catenin signaling pathway-related gene was analyzed to further uncover the regulatory mechanism of miR-27a. The osteosarcoma tissues and cells exhibited elevated miR-27 expression and reduced SFRP1 expression. SFRP1 was verified to be a target gene of miR-27a. Meanwhile, silenced miR-27a inhibited proliferation and invasion of human osteosarcoma cells. Finally, silencing miR-27a inhibited the activation of Wnt/β-catenin signaling pathway, evidenced by reduced β-catenin expression. Our study draws a conclusion that silencing miR-27a dampens osteosarcoma progression, which might be achieved through the inactivation of the Wnt/β-catenin signaling pathway by up-regulating SFRP1. |
| تدمد: | 1573-4935 0144-8463 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da05592d86039979a907b8f7921b9ed0Test https://doi.org/10.1042/bsr20182366Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....da05592d86039979a907b8f7921b9ed0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15734935 01448463 |
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