The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies
| العنوان: | The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies |
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| المؤلفون: | Dita Strachotová, Sandra Paiva, Helena Kučerová, Libuše Váchová, Margarida Casal, Otakar Hlaváček, Sandra Mota, Zdena Palková |
| المساهمون: | Repositório Científico do Instituto Politécnico do Porto |
| المصدر: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| بيانات النشر: | Portland Press Ltd., 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Monocarboxylic Acid Transporters, Saccharomyces cerevisiae Proteins, Saccharomyces cerevisiae, Carboxylic Acids, Biological Transport, Active, Biochemistry, Diffusion, Gene Knockout Techniques, 03 medical and health sciences, Ammonia, chemistry.chemical_compound, Lactic Acid, Molecular Biology, Transcription factor, 030304 developmental biology, 0303 health sciences, Symporters, biology, 030302 biochemistry & molecular biology, Transporter, Cell Biology, biology.organism_classification, Yeast, Lactic acid, Repressor Proteins, Membrane, chemistry, Reprogramming, Signal Transduction |
| الوصف: | On solid substrates, yeast colonies pass through distinct developmental phases characterized by the changes in pH of their surroundings from acidic to nearly alkaline and vice versa. At the beginning of the alkali phase colonies start to produce ammonia, which functions as a quorum-sensing molecule inducing the reprogramming of cell metabolism. Such reprogramming includes, among others, the activation of several plasma membrane transporters and is connected with colony differentiation. In the present study, we show that colony cells can use two transport mechanisms to import lactic acid: a ‘saturable’ component of the transport, which requires the presence of a functional Jen1p transporter, and a ‘non-saturable’ component (diffusion) that is independent of Jen1p. During colony development, the efficiency of both transport components changes similarly in central and outer colonial cells. Although the lactate uptake capacity of central cells gradually decreases during colony development, the lactate uptake capacity of outer cells peaks during the alkali phase and is also kept relatively high in the second acidic phase. This lactate uptake profile correlates with the localization of the Jen1p transporter to the plasma membrane of colony cells. Both lactic acid uptake mechanisms are diminished in sok2 colonies where JEN1 expression is decreased. The Sok2p transcription factor may therefore be involved in the regulation of non-saturable lactic acid uptake in yeast colonies. |
| وصف الملف: | application/pdf |
| تدمد: | 1470-8728 0264-6021 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4272794e7c5a741ed3bfb261584d6498Test https://doi.org/10.1042/bj20120312Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4272794e7c5a741ed3bfb261584d6498 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14708728 02646021 |
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