دورية أكاديمية
Endoplasmic reticulum resident proteins of normal human dermal fibroblasts are the major targets for oxidative stress induced by hydrogen peroxide
| العنوان: | Endoplasmic reticulum resident proteins of normal human dermal fibroblasts are the major targets for oxidative stress induced by hydrogen peroxide |
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| المؤلفون: | VLIES, Dennis van der, PAP, Eward H.W., POST, Jan Andries, CELIS, Julio E., WIRTZ, Karel W.A. |
| المصدر: | Biochemical Journal ; volume 366, issue 3, page 825-830 ; ISSN 0264-6021 1470-8728 |
| بيانات النشر: | Portland Press Ltd. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Cell Biology, Molecular Biology, Biochemistry |
| الوصف: | The membrane-permeable fluorescein-labelled tyramine conjugate (acetylTyrFluo) was used to identify the proteins of normal human dermal fibroblasts most susceptible to oxidation by hydrogen peroxide [Van der Vlies, Wirtz and Pap (2001) Biochemistry 40, 7783—7788]. By exposing the cells to H2O2 (0.1mM for 10min), TyrFluo was covalently linked to target proteins. TyrFluo-labelled and [35S]Met-labelled cell lysates were mixed and subjected to two-dimensional PAGE. After Western blotting the 35S-labelled proteins were visualized by autoradiography and the TyrFluo-labelled proteins by using anti-fluorescein antibody. The TyrFluo-labelled proteins were matched with the 35S-labelled proteins and identified by comparison with our mastermap of proteins. Protein disulphide isomerase (PDI), IgG-binding protein (BiP), calnexin, endoplasmin and glucose-regulated protein 58 (endoplasmic reticulum protein 57/GRP58) were identified as targets of oxidation. All these proteins reside in the endoplasmic reticulum and are part of the protein folding machinery. In agreement, confocal laser scanning microscopy showed co-localization of TyrFluo-labelled proteins and the KDEL receptor ERD-2, a marker for the endoplasmic reticulum. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1042/bj20020618 |
| الإتاحة: | https://doi.org/10.1042/bj20020618Test https://portlandpress.com/biochemj/article-pdf/366/3/825/709818/bj3660825.pdfTest |
| رقم الانضمام: | edsbas.42ADC3B4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1042/bj20020618 |
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