Control and production of leukotriene B4 in rat tumour and testicular Leydig cells
| العنوان: | Control and production of leukotriene B4 in rat tumour and testicular Leydig cells |
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| المؤلفون: | B. A. Cooke, M. H. F. Sullivan |
| المصدر: | Biochemical Journal. 230:821-824 |
| بيانات النشر: | Portland Press Ltd., 1985. |
| سنة النشر: | 1985 |
| مصطلحات موضوعية: | Male, endocrine system, medicine.medical_specialty, Leukotriene B4, Catechols, Testicle, Biology, Biochemistry, chemistry.chemical_compound, Testicular Neoplasms, Internal medicine, medicine, Animals, Masoprocol, Testosterone, Lipoxygenase Inhibitors, Molecular Biology, Calcimycin, Leukotriene, Leydig cell, Leydig Cells, Radioimmunoassay, Cell Biology, Luteinizing Hormone, Rats, Nordihydroguaiaretic acid, Endocrinology, medicine.anatomical_structure, chemistry, Luteinizing hormone, Research Article, Leydig Cell Tumor |
| الوصف: | As part of an investigation into the role of leukotrienes in steroidogenesis, the formation of leukotriene B4 was investigated in purified Leydig cells from rat testes and from a tumour by using a sensitive radioimmunoassay. Detectable levels were found in both Leydig cell types (70 pg/10(6) cells) and these remain unchanged during incubation for 60 min at 32 degrees C. Addition of the Ca2+ ionophore A23187 increased LTB4 production more than 6-fold within 10 min whereas steroidogenesis was not increased until after 20 min. In the presence of luteinizing hormone or luteinizing hormone releasing hormone agonist no increase in LTB4 was detected in the testis Leydig cells whereas luteinizing hormone stimulated testosterone production from 3.2 +/- 0.1 to 148.9 +/- 7.5 ng/10(6) cells during the same time period. Similar results were obtained with the tumour Leydig cells. The LTB4 was found to be rapidly secreted by the cells in all experiments. The basal and A23187-stimulated levels were inhibited by nordihydroguaiaretic acid, a lipoxygenase inhibitor. It is concluded that LTB4 is produced in Leydig cells and can be stimulated by high calcium levels, but that it is probably not required for the control of steroidogenesis. |
| تدمد: | 1470-8728 0264-6021 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92e4ab538d137673cb58b0ffb684b318Test https://doi.org/10.1042/bj2300821Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....92e4ab538d137673cb58b0ffb684b318 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14708728 02646021 |
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