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مصطلحات موضوعية: Delta, chemistry.chemical_classification, biology, Mutagenesis, Biochemistry, Sphingolipid, Glutamine, Enzyme, Fatty acid desaturase, chemistry, biology.protein, Consensus sequence, Histidine
الوصف: The consensus sequence of the third histidine box of a range of Delta(5), Delta(6), Delta(8) and sphingolipid desaturases differs from that of the membrane-bound non-fusion Delta(12) and Delta(15) desaturases in the presence of glutamine instead of histidine. We have used site-directed mutagenesis to determine the importance of glutamine and other residues of the third histidine box and created a chimaeric enzyme to determine the ability of the Cyt b(5) fusion domain from the plant sphingolipid desaturase to substitute for the endogenous domain of the Delta(6) desaturase.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44f38f61c23b09e513c7434bd8f24e43Test
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المؤلفون: Alex F. Drake, Arthur S. Tatham, Peter R. Shewry
مصطلحات موضوعية: Circular dichroism, Glutens, Proline, Protein Conformation, Stereochemistry, Glutamine, Biochemistry, Hydrophobic effect, Protein structure, Urea, Amino Acid Sequence, Molecular Biology, Protein secondary structure, Plant Proteins, Ethanol, Chemistry, Circular Dichroism, Protein primary structure, Hordeum, Cell Biology, Crystallography, Hordein, Seeds, Research Article
الوصف: A combination of c.d. spectroscopy and computer prediction is used to show that C hordein has an unusual secondary structure with an absence of alpha-helix and beta-sheet, but the presence of regularly repeated beta-turns. This is associated with a repetitive primary structure based mainly on blocks of eight residues. Similar spectral changes occurred when the protein was heated from 6 to 86 degrees C in aq. 70% (v/v) ethanol or dissolved in increasing concentrations (50-100%, v/v) of trifluoroethanol in water. The studies indicated that the conformation is stabilized by strong hydrophobic interactions and by extensive hydrogen-bonding.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9a6e5a378752349a41f401dac7a4750Test
