Molecular cloning of an actinobacterial-type ClpS gene from Celosia cristata expression library
| العنوان: | Molecular cloning of an actinobacterial-type ClpS gene from Celosia cristata expression library |
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| المؤلفون: | B. BaghbanKohnehrouz, A. Gholizadeh, M. H. Faizi, H. C. Kapoor |
| المصدر: | Russian Journal of Plant Physiology. 55:521-529 |
| بيانات النشر: | Pleiades Publishing Ltd, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Genetics, biology, Celosia cristata, Chaperone (protein), Complementary DNA, Protein domain, biology.protein, Coding region, Plant Science, Molecular cloning, biology.organism_classification, Gene, Peptide sequence |
| الوصف: | In proteobacterial cytosol, ClpS protein is known as a molecular adaptor for substrate selectivity and proteolytic activity of the ATP-dependent chaperone-protease complex, ClpAP. ClpA-related ClpS is a small protein usually encoded immediately upstream of ClpA in the genome of proteobacteria. Recent bioinformatics analysis has revealed the presence of cyanobacterial-type ClpS or ClpC-related ClpS in organisms lacking ClpA, including all the plant species sequenced to date. Here we report the identification of an actinobacterial homologue of the ClpS (possibly Clp-related) gene from a plant system. A cDNA, spanning 566 bp with a complete coding region corresponding to 132 amino acids, was isolated from a Celosia cristata expression library constructed on a λ TriplEX2 vector. This cDNA product was considered to be an ATP-dependent Clp protease adaptor and was designated as Celosia actinobacterial-type ClpS, since it contains a highly conserved domain belonging to the ClpS family of proteins from actinobacteria. Celosia ClpS is about 80% identical to actinobacterial ClpS proteins in its overall deduced amino acid sequence. Based on this finding, we may define a novel target of ATP-dependent Clp complex in a plant system or speculate the presence of a second type of molecular chaperone besides ClpC in plants, as predicted for actinobacteria. |
| تدمد: | 1608-3407 1021-4437 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::2243a21ba9e04e42b66d88415b1e89b6Test https://doi.org/10.1134/s1021443708040146Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........2243a21ba9e04e42b66d88415b1e89b6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16083407 10214437 |
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