التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of biochemical constituents and inhibitory effect of tea clone 100 on colorectal cancer cell line HCT-116. |
| المؤلفون: |
Bondarian, Fereydoon1, Ebrahimi, Asa2 dr.asaebrahimi@gmail.com, Mahjoubi, Frouzandeh3, Hervan, Eslam Majidi1, Gonbad, Reza Azadi4 |
| المصدر: |
Tropical Journal of Pharmaceutical Research. Jun2018, Vol. 17 Issue 6, p1033-1041. 9p. |
| مصطلحات موضوعية: |
*COLON cancer, *CANCER cells, *EPIGALLOCATECHIN gallate, *POLYPHENOLS, *CATECHIN |
| مستخلص: |
Purpose: To evaluate the total content of polyphenols and the free radical scavenging activity of three different extracts of three types of tea clone 100 (black, green and white), and their anti-proliferative effects on colorectal cancer. Methods: Five major polyphenols, viz, (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate and (-)-epigallocatechin gallate, were identified using thin layer chromatography (TLC). Catechins were quantified by high performance liquid chromatography (HPLC). Antioxidant activity was measured by DPPH radical scavenging method, while 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) assay was employed for the determination of cell viability of colon cell line HCT-116 after 24 and 48 h. Results: The aqueous methanol (70 %) extract of white tea yielded the highest amount of polyphenols (36.67 ± 0.54 mg GAE/g dry weight). The DPPH radical scavenging activity of white tea was 71.74 ± 0.42 %, and it produced high anti-proliferation activity against colorectal cancer cell line HCT-116 (86.06 ± 0.54 %). Conclusion: White tea extract possesses high DPPH radical scavenging activity, and exerts good antiproliferative effects against colorectal cancer cell line HCT-116, most likely due to its catechin content. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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