Tenascin-X Induces Cell Detachment through p38 Mitogen-Activated Protein Kinase Activation
| العنوان: | Tenascin-X Induces Cell Detachment through p38 Mitogen-Activated Protein Kinase Activation |
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| المؤلفون: | Hiroshi Maita, Ken-ichi Matsumoto, Hiroyoshi Ariga, Shinpei Fujie |
| المصدر: | Biological and Pharmaceutical Bulletin. 32:1795-1799 |
| بيانات النشر: | Pharmaceutical Society of Japan, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Focal Adhesion Protein-Tyrosine Kinases/metabolism, Signal Transduction, p38 mitogen-activated protein kinases, Pharmaceutical Science, Cell Adhesion/physiology, p38 Mitogen-Activated Protein Kinases, Phosphorylation, Tenascin X, Focal adhesion, Mice, p38 Mitogen-Activated Protein Kinases/metabolism, Cell Adhesion, Animals, Cell adhesion, Cells, Cultured, Pharmacology, Extracellular Matrix Proteins, Extracellular Matrix Proteins/metabolism, biology, tenascin-X, p38 mitogen-activated protein kinase, cell detachment, Kinase, Tenascin, Fibroblasts/metabolism, General Medicine, Fibroblasts, Molecular biology, Cell biology, Focal Adhesion Protein-Tyrosine Kinases, Tenascin/metabolism, Mitogen-activated protein kinase, biology.protein, Signal transduction |
| الوصف: | Extracellular matrix glycoprotein tenascin-X (TNX) is the largest member of the tenascin family. In this study, we investigated the adhesive properties of TNX and the signaling pathway to be induced to mouse fibroblast L cells on TNX substrate. Approximately 45% of evaluable cells used in the cell adhesion assay were attached to purified TNX but did not spread and were rounded on TNX. The remaining 55% of cells were detached from the TNX substrate and were floating in the conditioned medium. In rounded cells on TNX, phosphorylation of focal adhesion kinase (FAK) was diminished compared with that in cells on control phosphate buffered saline (PBS). To better understand the pathways that lead to the detachment of cells on the TNX substrate, we examined phosphorylation of p38 mitogen-activated protein (MAP) kinase. Phosphorylation of p38 MAP kinase was observed in the rounded cells on TNX in a dose-dependent manner, and the maximum effect was observed at 30 min on TNX. Inhibition of p38 MAP kinase alpha expression by RNA interference partially suppressed the TNX-induced cell detachment. These results suggest that the p38 MAP kinase is a major mediator of TNX-induced cell detachment. |
| وصف الملف: | application/pdf |
| تدمد: | 1347-5215 0918-6158 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99c10c26abb0c03bcb725b9d939741a3Test https://doi.org/10.1248/bpb.32.1795Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....99c10c26abb0c03bcb725b9d939741a3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13475215 09186158 |
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