دورية أكاديمية
Growth hormone and somatostatin gene expression in pituitary adenomas with active acromegaly and minimal plasma growth hormone elevation
| العنوان: | Growth hormone and somatostatin gene expression in pituitary adenomas with active acromegaly and minimal plasma growth hormone elevation |
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| المؤلفون: | Pagesy, Patrick, Li, Jacques Y., Rentier-Delrue, Françoise, Delalande, Olivier, Le Bouc, Yves, Kujas, Michèle, Bression, Dominique, Martial, Joseph, Peillon, Françoise |
| المصدر: | Acta Endocrinologica, 122 (6), 745-752 (1990) |
| بيانات النشر: | Periodica |
| سنة النشر: | 1990 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Growth hormone, somatostatin, pituitary adenomas, Life sciences, Biochemistry, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | peer reviewed ; Some patients with active acromegaly have elevated plasma IGF-I concentrations with only minimal elevation of plasma GH. We compared adenomatous GH and SRIH expression in 3 such patients (patients No. 1, 2 and 3; basal plasma GH level < 4 µg/l) and in 3 acromegalic patients with high basal plasma GH level (patients No. 4, 5 and 6; 51.7 ± 16.1 µg/l, mean ± SEM). By immunocytochemistry, all the tumours proved to be somatotropic adenomas. At the ultrastructural level, signs of low secretory activity were observed in adenomas from patients No. 2 and 3. Perifused adenoma cells of patients No. 1, 2 and 3 released very little GH compared with those of patients No. 4, 5 and 6 (1± 0.37 vs 51.5± 34.1 µg · (10–6 cells) · min–1, p< 0.001). Adenoma SRIH content was 65.7 and 30.6 pg/mg proteins in patients No. 1 and 2, whereas it was undetectable in the others (patients No. 4, 5 and 6). Northern blot analysis showed that the GH gene was poorly expressed in the adenomas from patients No. 1, 2 and 3 compared with the adenomas from patients No. 4, 5 and 6. SRIH mRNA was detected in all 6 adenomas. However, the signal was more intense in the adenomas from patients No. 1, 2 and 3 than in those from patients No. 4, 5 and 6. In conclusion, because of the variability of the biosynthetic and secretory potential of the somatotropic adenomas, patients harbouring this type of pituitary tumours can exhibit a wide range of plasma GH levels. In acromegaly with minimal elevation of plasma GH, the synthesis of SRIH by the adenoma cells themselves could play a role in the inhibition of GH expression. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0001-5598 |
| العلاقة: | http://eje-online.org/cgi/content/abstract/122/6/745Test; urn:issn:0001-5598; https://orbi.uliege.be/handle/2268/20100Test; info:hdl:2268/20100; scopus-id:2-s2.0-0025359199; info:pmid:1695808 |
| DOI: | 10.1530/acta.0.1220745 |
| الإتاحة: | https://doi.org/10.1530/acta.0.1220745Test https://orbi.uliege.be/handle/2268/20100Test |
| رقم الانضمام: | edsbas.F35CB6A1 |
| قاعدة البيانات: | BASE |
| تدمد: | 00015598 |
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| DOI: | 10.1530/acta.0.1220745 |