دورية أكاديمية
Evidence for separate effects of U73122 on phospholipase C and calcium channels in human platelets
| العنوان: | Evidence for separate effects of U73122 on phospholipase C and calcium channels in human platelets |
|---|---|
| المؤلفون: | PULCINELLI, FABIO MARIA, GAZZANIGA, Pierpaolo, Paolo Gresele, Margherita Bonuglia |
| المساهمون: | Pulcinelli, FABIO MARIA, Paolo, Gresele, Margherita, Bonuglia, Gazzaniga, Pierpaolo |
| بيانات النشر: | PERGAMON-ELSEVIER SCIENCE LTD |
| سنة النشر: | 1998 |
| المجموعة: | Sapienza Università di Roma: CINECA IRIS |
| مصطلحات موضوعية: | calcium channel, calcium mobilization, phospholipase c, platelets |
| الوصف: | U73122 ({1-[6-((17 beta-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)exyl]-1H-pyrrole-2,5-dione}) is generally used as a selective inhibitor of phospholipase C (PLC) and the related rise in cytosolic Ca2+. Recently, by using hepatocytes, it was suggested that its action sites are different for PLC activation and increase in Ca2+ concentration. To verify whether U73122 has different sites for inhibiting PLC activation and calcium responses in human platelets, aggregation, Mn2+ influx, cytosolic Ca2+ increase and PLC activation were studied in response to thrombin and the synthetic agonist of the thromboxane receptor U46619 (9,11-dideoxy-9 alpha,11 alpha-methanoepoxyprostaglandin F-2 alpha). With both agonists, U73122 inhibited aggregation, Mn2+ influx and the enhancement of cytosolic calcium at concentrations of 2 mu M or lower, while 10 mu M was necessary to inhibit PLC activation. Our results suggested that U73122 is much more active in antagonizing Ca2+ channels, both the intracellular ones, which are activated by formation of inositol 1,4,5 P-3 and those present on plasma membrane, than in reducing the activation of PLC. BIOCHEM PHARMACOL 56;11:1481-1484, 1998. (C) 1998 Elsevier Science Inc. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/9827580; info:eu-repo/semantics/altIdentifier/wos/WOS:000077035100009; volume:56; issue:11; firstpage:1481; lastpage:1484; numberofpages:4; journal:BIOCHEMICAL PHARMACOLOGY; http://hdl.handle.net/11573/105768Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0031770306; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000077035100009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396aTest; http://www.scopus.com/inward/record.url?eid=2-s2.0-0031770306&partnerID=65&md5=c7e74eb99056cb31fc66c323c5bce0baTest |
| DOI: | 10.1016/s0006-2952(98)00146-4 |
| الإتاحة: | https://doi.org/10.1016/s0006-2952Test(98)00146-4 http://hdl.handle.net/11573/105768Test http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000077035100009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396aTest http://www.scopus.com/inward/record.url?eid=2-s2.0-0031770306&partnerID=65&md5=c7e74eb99056cb31fc66c323c5bce0baTest |
| رقم الانضمام: | edsbas.3E463EA9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/s0006-2952(98)00146-4 |
|---|