دورية أكاديمية
A pyroptosis gene-based prognostic model for predicting survival in low-grade glioma
| العنوان: | A pyroptosis gene-based prognostic model for predicting survival in low-grade glioma |
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| المؤلفون: | Hua Wang, Lin Yan, Lixiao Liu, Xianghe Lu, Yingyu Chen, Qian Zhang, Mengyu Chen, Lin Cai, Zhang’an Dai |
| المصدر: | PeerJ, Vol 11, p e16412 (2023) |
| بيانات النشر: | PeerJ Inc., 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine LCC:Biology (General) |
| مصطلحات موضوعية: | Pyroptosis, Low-grade gliomas, Risk scoring model, Prognosis, Individualized treatment, Medicine, Biology (General), QH301-705.5 |
| الوصف: | Background Pyroptosis, a lytic form of programmed cell death initiated by inflammasomes, has been reported to be closely associated with tumor proliferation, invasion and metastasis. However, the roles of pyroptosis genes (PGs) in low-grade glioma (LGG) remain unclear. Methods We obtained information for 1,681 samples, including the mRNA expression profiles of LGGs and normal brain tissues and the relevant corresponding clinical information from two public datasets, TCGA and GTEx, and identified 45 differentially expressed pyroptosis genes (DEPGs). Among these DEPGs, nine hub pyroptosis genes (HPGs) were identified and used to construct a genetic risk scoring model. A total of 476 patients, selected as the training group, were divided into low-risk and high-risk groups according to the risk score. The area under the curve (AUC) values of the receiver operating characteristic (ROC) curves verified the accuracy of the model, and a nomogram combining the risk score and clinicopathological characteristics was used to predict the overall survival (OS) of LGG patients. In addition, a cohort from the Gene Expression Omnibus (GEO) database was selected as a validation group to verify the stability of the model. qRT-PCR was used to analyze the gene expression levels of nine HPGs in paracancerous and tumor tissues from 10 LGG patients. Results Survival analysis showed that, compared with patients in the low-risk group, patients in the high-risk group had a poorer prognosis. A risk score model combining PG expression levels with clinical features was considered an independent risk factor. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that immune-related genes were enriched among the DEPGs and that immune activity was increased in the high-risk group. Conclusion In summary, we successfully constructed a model to predict the prognosis of LGG patients, which will help to promote individualized treatment and provide potential new targets for immunotherapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2167-8359 |
| العلاقة: | https://peerj.com/articles/16412.pdfTest; https://peerj.com/articles/16412Test/; https://doaj.org/toc/2167-8359Test |
| DOI: | 10.7717/peerj.16412 |
| الوصول الحر: | https://doaj.org/article/a1e3985ef131408e8b3f012993cecb43Test |
| رقم الانضمام: | edsdoj.1e3985ef131408e8b3f012993cecb43 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21678359 |
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| DOI: | 10.7717/peerj.16412 |