مورد إلكتروني
Depot-specific messenger RNA expression of 11 beta-hydroxysteroid dehydrogenase type 1 and leptin in adipose tissue of children and adults
| العنوان: | Depot-specific messenger RNA expression of 11 beta-hydroxysteroid dehydrogenase type 1 and leptin in adipose tissue of children and adults |
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| بيانات النشر: | Pediatrik Kirurgi Institutionen för folkhälsa och klinisk medicin Children's Research Institute of Nanjing Medical University, Nanjing, China Department of Pediatric Surgery, University Children's Hospital, Uppsala, Sweden London : Macmillan 2007 |
| تفاصيل مُضافة: | Li, X. Lindquist, Susanne Chen, R. Myrnäs, Torbjörn Angsten, G. Olsson, Tommy Hernell, Olle |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Objective: To compare expression of messenger RNA (mRNA) coding for the cortisol regenerating enzyme 11 -hydroxysteroid dehydrogenase type 1 (11-HSD1), and the adipocytokines leptin and resistin in paired biopsies of subcutaneous adipose tissue (SC) and omental adipose tissue (OM) from children. Design: Paired biopsies (SC and OM) were obtained from 54 children (age 0.17–16 years, body mass index (BMI) 12.5–28.3 kg/m2, BMI standard deviation score (SDS) -2.5–4.5) and 16 adults (age 27–79 years, BMI 19–46 kg/m2) undergoing open abdominal surgery. mRNA levels of 11-HSD1, leptin and resistin were measured using quantitative real-time polymerase chain reaction (PCR). Results: 11-HSD1 mRNA level was higher in OM than in SC (P<0.05), whereas leptin mRNA was higher in SC than in OM (P<0.001). There was no difference in the resistin mRNA level between SC and OM. These results were consistent in children and adults. In children, 11-HSD1 mRNA in SC was positively associated with BMI SDS (P<0.05), whereas in OM it was positively associated with age (P<0.05). The association between 11-HSD1 expression and age remained significant after adjustment for BMI SDS and gender. Leptin mRNA was positively associated with BMI SDS (SC:P<0.001, OM: P<0.001) but not with age in children. In multiple regression analyses, including anthropometric variables and age, BMI SDS was independently associated with mRNA levels of 11-HSD1 (P<0.05) and leptin (P<0.001) in |
| مصطلحات الفهرس: | 11-beta-Hydroxysteroid Dehydrogenase Type 1/*genetics, Adipose Tissue/enzymology/*pathology, Adolescent, Adult, Aged, Aging/genetics, Anthropometry, Body Mass Index, Child, Child; Preschool, Female, Humans, Infant, Leptin/*genetics, Male, Middle Aged, Obesity/*genetics, Overweight/*genetics, RNA; Messenger/*genetics, Regression Analysis, Resistin/*genetics, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1038.sj.ijo.0803470 |
| URL: | International Journal of Obesity, 0307-0565, 2007, 31:5, s. 820-828 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | English |
| أرقام أخرى: | UPE oai:DiVA.org:umu-10073 doi:10.1038/sj.ijo.0803470 PMID 17060929 Scopus 2-s2.0-34247494724 1234461535 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1234461535 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1038.sj.ijo.0803470 |
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