Immunohistochemical localization of periostin in human gingiva
| العنوان: | Immunohistochemical localization of periostin in human gingiva |
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| المؤلفون: | José A. Vega, Teresa Cobo, Laura Fernandez Solares, Juan Cobo, Alvaro J. Obaya, R. Cabo, Santiago Cal |
| المصدر: | European Journal of Histochemistry, Vol 59, Iss 3 (2015) Scopus European Journal of Histochemistry : EJH RUO. Repositorio Institucional de la Universidad de Oviedo instname |
| بيانات النشر: | PAGEPress Publications, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Male, Pathology, medicine.medical_specialty, Histology, Adolescent, Periodontal Ligament, human gingiva, Gingiva, Biophysics, Connective tissue, Vimentin, Periostin, Cytokeratin, Western blot, medicine, Humans, Periodontal fiber, lcsh:QH301-705.5, Original Paper, biology, medicine.diagnostic_test, Chemistry, Matricellular protein, Cell Biology, matricellular proteins, medicine.anatomical_structure, lcsh:Biology (General), biology.protein, Keratins, Immunohistochemistry, Female, Cell Adhesion Molecules |
| الوصف: | The periostin is a matricellular protein expressed in collagen-rich tissues including some dental and periodontal tissues where it is regulated by mechanical forces, growth factors and cytokines. Interestingly the expression of this protein has been found modified in different gingival pathologies although the expression of periostin in normal human gingiva was never investigated. Here we used Western blot and double immunofluorescence coupled to laser-confocal microscopy to investigated the occurrence and distribution of periostin in different segments of the human gingival in healthy subjects. By Western blot a protein band with an estimated molecular mass of 94 kDa was observed. Periostin was localized at the epithelial-connective tissue junction, or among the fibers of the periodontal ligament, and never co-localized with cytokeratin or vimentin thus suggesting it is an extracellular protein. These results demonstrate the occurrence of periostin in adult human gingiva; its localization suggests a role in the bidirectional interactions between the connective tissue and the epithelial cells, and therefore in the physiopathological conditions in which these interactions are altered. Â |
| تدمد: | 2038-8306 1121-760X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::927f988795642b059d3584b947fe63bfTest https://doi.org/10.4081/ejh.2015.2548Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....927f988795642b059d3584b947fe63bf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20388306 1121760X |
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