New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells
| العنوان: | New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells |
|---|---|
| المؤلفون: | Bianca D. Capaldo, Helen E. Abud, Qi Zhou, Shiri Blumenfeld, Karen Oliva, Paul J. McMurrick, Joshua Kie, Geoffrey J. Lindeman, Jane E. Visvader, Andrew L. Laslett, William J. McKinstry, Timothy E. Adams, Di Chen, Christian M. Nefzger, Jose M. Polo, Thierry Jarde, John D. Bentley, Jeanne F. Loring, Jack W. Lambshead, Yu-Chieh Wang, Xiaodong Liu, Tung Liang Chung, Fernando J. Rossello, Carmel M. O’Brien, Tram Phan, Amander T. Clark, Hun S. Chy |
| المصدر: | Stem Cells (Dayton, Ohio) Stem cells (Dayton, Ohio), vol 35, iss 3 |
| بيانات النشر: | Oxford University Press (OUP), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Technology, Cellular differentiation, Cell Culture Techniques, Embryoid body, Medical and Health Sciences, Embryonic Stem Cells/Induced Pluripotent Stem Cells, Mice, Monoclonal, Cell surface markers, Breast, Cell Self Renewal, Naive, Induced pluripotent stem cell, Colorectal, Cancer, Antibodies, Monoclonal, Cell Differentiation, Biological Sciences, Flow Cytometry, Cell biology, Surface, Antigens, Surface, Molecular Medicine, Human embryonic stem cells, Stem cell, Reprogramming, Pluripotent Stem Cells, Pluripotency, Human iPS cells, Immunology, Down-Regulation, Biology, Antibodies, 03 medical and health sciences, Animals, Humans, Antigens, Cell potency, Embryoid Bodies, Mesenchymal stem cell, Membrane Proteins, Cell Biology, Hematopoietic Stem Cells, Molecular biology, Embryonic stem cell, 030104 developmental biology, Monoclonal antibodies, Octamer Transcription Factor-3, Developmental Biology |
| الوصف: | The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types. |
| وصف الملف: | application/pdf |
| تدمد: | 1549-4918 1066-5099 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04eabc3e899b2e23c30bdad68fe28fd5Test https://doi.org/10.1002/stem.2558Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....04eabc3e899b2e23c30bdad68fe28fd5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15494918 10665099 |
|---|