Determination and Pharmacokinetic Study of Pirfenidone in Rat Serum by High-Performance Thin-Layer Chromatography
| العنوان: | Determination and Pharmacokinetic Study of Pirfenidone in Rat Serum by High-Performance Thin-Layer Chromatography |
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| المؤلفون: | Rupesh V. Chikhale, Sonali G. Thorat |
| المصدر: | Journal of Chromatographic Science. 54:1115-1119 |
| بيانات النشر: | Oxford University Press (OUP), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Pyridones, Calibration curve, Administration, Oral, 01 natural sciences, Lower limit, Analytical Chemistry, Acetone, Rats, Sprague-Dawley, 03 medical and health sciences, Pharmacokinetics, Limit of Detection, Oral administration, medicine, Animals, Protein precipitation, High performance thin layer chromatography, Retardation factor, Analgesics, Chromatography, Chemistry, 010401 analytical chemistry, Reproducibility of Results, General Medicine, Pirfenidone, Rats, 0104 chemical sciences, 030104 developmental biology, Calibration, Chromatography, Thin Layer, Densitometry, Toluene, medicine.drug |
| الوصف: | A rapid, sensitive and selective high-performance thin-layer chromatography (HPTLC) method was developed and validated for the determination and pharmacokinetics of pirfenidone in rat serum. One-step protein precipitation by methanol is reported, and serum samples were separated by HPTLC using a simple mobile phase of toluene-methanol in the ratio of 8:2. The retardation factor of pirfenidone in the serum sample was 0.45 with the detection performed at 315 nm. The calibration curve was linear over the range of 100-1,200 ng/spot with a lower limit of quantitation of 40 ng/spot. The mean recovery of pirfenidone in serum was in the range of 70.6-75.8%, and intra-day and inter-day precision were both |
| تدمد: | 1945-239X 0021-9665 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ebeeb54c51ee810a0fe4b6f95234ae4Test https://doi.org/10.1093/chromsci/bmw067Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0ebeeb54c51ee810a0fe4b6f95234ae4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1945239X 00219665 |
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