Enhanced expression of IFI16 and RIG-I in human third-trimester placentas following HSV-1 infection
| العنوان: | Enhanced expression of IFI16 and RIG-I in human third-trimester placentas following HSV-1 infection |
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| المؤلفون: | Mirosława Studzińska, Jarosław Kalinka, Agnieszka Jabłońska, Patrycja Suski, Edyta Paradowska |
| المصدر: | Clinical and Experimental Immunology. 193:255-263 |
| بيانات النشر: | Oxford University Press (OUP), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, 0301 basic medicine, Biopsy, Placenta, Pregnancy Trimester, Third, medicine.medical_treatment, Immunology, Herpesvirus 1, Human, Biology, Andrology, 03 medical and health sciences, Organ Culture Techniques, 0302 clinical medicine, Immune system, Pregnancy, Interferon, medicine, Humans, Immunology and Allergy, Pregnancy Complications, Infectious, Receptors, Immunologic, Cells, Cultured, Cesarean Section, Decidua, Nuclear Proteins, Trophoblast, Herpes Simplex, Original Articles, Phosphoproteins, Immunity, Innate, Up-Regulation, 030104 developmental biology, medicine.anatomical_structure, Cytokine, embryonic structures, TLR3, DEAD Box Protein 58, Chorionic villi, Female, 030215 immunology, medicine.drug |
| الوصف: | Summary The innate immune response in the placenta depends on the ability of maternal immune cells and fetal trophoblast cells to detect and eliminate invading pathogens through germline-encoded pattern recognition receptors (PRRs). In the present study, we analysed the transcripts and protein expression of interferon (IFN)-inducible protein (IFI)16, melanoma differentiation-associated protein 5 (MDA5), RIG-I-like receptor (RIG-I) and Toll-like receptor (TLR)-3 in third-trimester human placentas and investigated cytokine profiles generated during herpes simplex type 1 (HSV-1) infection. Decidual and chorionic villous biopsies (38–42 weeks of gestation) were obtained from healthy women immediately after a caesarean section. The expression of the DDX58 (RIG-I), IFIH1 (MDA5), IFI16 and TLR3 transcripts was measured using quantitative real-time polymerase chain reaction (qRT–PCR). Extracellular cytokine and PRRs levels were then quantified by enzyme-linked immunosorbent assays (ELISAs). All examined PRRs genes, including DDX58, IFIH1, IFI16 and TLR3, were expressed constitutively at the mRNA and protein levels in the placental biopsies. The concentration of the IFI16 protein was increased in HSV-1-infected decidual and chorionic villous explants compared to those of mock-infected tissues (P = 0·029). Higher protein expression levels of RIG-I in both the maternal and fetal parts of the placenta were found (P = 0·009 and P = 0·004, respectively). In addition, increased production of IFN-β by HSV-1-infected tissues was noticed (P = 0·004 for decidua, P = 0·032 for chorionic villi). No significant differences in the IFN-α, interleukin (IL)-6 and IL-8 levels were found. These results showed that HSV-1 infection can enhance the expression of IFI16 and RIG-I proteins in the human term placenta. |
| تدمد: | 1365-2249 0009-9104 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::107270c9609e0aaf0a94cc3b78a3f685Test https://doi.org/10.1111/cei.13143Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....107270c9609e0aaf0a94cc3b78a3f685 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652249 00099104 |
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