A 22-Amino Acid Synthetic Peptide Corresponding to the Second Extracellular Loop of Rat Occludin Perturbs the Blood-Testis Barrier and Disrupts Spermatogenesis Reversibly In Vivo1
| العنوان: | A 22-Amino Acid Synthetic Peptide Corresponding to the Second Extracellular Loop of Rat Occludin Perturbs the Blood-Testis Barrier and Disrupts Spermatogenesis Reversibly In Vivo1 |
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| المؤلفون: | Meng-yun Mo, Dolores D. Mruk, C. Yan Cheng, Will M. Lee, Nancy P. Y. Chung |
| المصدر: | Biology of Reproduction. 65:1340-1351 |
| بيانات النشر: | Oxford University Press (OUP), 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | chemistry.chemical_classification, endocrine system, Basal ectoplasmic specialization, Tight junction, urogenital system, Peptide, Cell Biology, General Medicine, Biology, Occludin, Sertoli cell, Cell biology, Amino acid, medicine.anatomical_structure, Reproductive Medicine, chemistry, Biochemistry, Extracellular, medicine, Blood–testis barrier |
| الوصف: | When Sertoli cells were cultured in vitro on Matrigel-coated bicameral units, the assembly of the inter-Sertoli tight junction (TJ) permeability barrier correlated with an induction of occludin expression. Inclusion of a 22-amino acid peptide, NH2-GSQIYTICSQFYTPGGTGLYVD-COOH, corresponding to residues 209–230 in the second extracellular loop of rat occludin, at 0.2–4 μM into Sertoli cell cultures could perturb the assembly of Sertoli TJs dose-dependently and reversibly. This peptide apparently exerts its effects by interfering with the homotypic interactions of two occludin molecules between adjacent Sertoli cells at the sites of TJs, thereby disrupting TJs, which, in turn, causes a decline in transepithelial electrical resistance across the Sertoli cell epithelium. When similar experiments were performed using a 22-amino acid myotubularin peptide, NH2-TKVNERYELCDTYPALLAVPAN-COOH (residues 156–177), no effects on the assembly of inter-Sertoli TJs in vitro were noted. When a single dose of this sy... |
| تدمد: | 1529-7268 0006-3363 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::98e646b61525a53d7c2aeb1ca86feb02Test https://doi.org/10.1095/biolreprod65.5.1340Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........98e646b61525a53d7c2aeb1ca86feb02 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15297268 00063363 |
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