QUASIMODO1 Encodes a Putative Membrane-Bound Glycosyltransferase Required for Normal Pectin Synthesis and Cell Adhesion in Arabidopsis
| العنوان: | QUASIMODO1 Encodes a Putative Membrane-Bound Glycosyltransferase Required for Normal Pectin Synthesis and Cell Adhesion in Arabidopsis |
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| المؤلفون: | Marie-Thérèse Leydecker, Grégory Mouille, Joël Talbotec, Hoai-Nam Truong, Edouard Leboeuf, Fabienne Granier, Herman Höfte, Sophie Bouton, Marc Lahaye |
| المساهمون: | Unité de recherche Nutrition Azotée des Plantes (URNAP), Institut National de la Recherche Agronomique (INRA), Laboratoire de biologie cellulaire et moléculaire, Unité de recherche Génétique et amélioration des plantes (GAP), Laboratoire de biochimie et technologie des glucides |
| المصدر: | The Plant cell The Plant cell, American Society of Plant Biologists (ASPB), 2002, 14 (10), pp.2577-2590. ⟨10.1105/tpc.004259⟩ |
| بيانات النشر: | Oxford University Press (OUP), 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | 0106 biological sciences, Pectin, [SDV]Life Sciences [q-bio], Mutant, Arabidopsis, polysaccharides, Fluorescent Antibody Technique, elongation, deficient, Plant Science, Plant Roots, 01 natural sciences, Cell Wall, Gene Expression Regulation, Plant, wall, Phylogeny, mutants, 0303 health sciences, biology, Hexuronic Acids, alignment, Phenotype, rhamnogalacturonan-ii, Biochemistry, Pectins, Plant Shoots, Research Article, food.ingredient, Gene Expression Regulation, Enzymologic, Cell wall, 03 medical and health sciences, food, homogalacturonan, Glycosyltransferase, Cell Adhesion, thaliana, Cell adhesion, Gene, 030304 developmental biology, Arabidopsis Proteins, Wild type, Glycosyltransferases, Membrane Proteins, Cell Biology, biology.organism_classification, Mutation, biology.protein, biosynthesis, 010606 plant biology & botany |
| الوصف: | International audience; Pectins are a highly complex family of cell wall polysaccharides. As a result of a lack of specific mutants, it has been difficult to study the biosynthesis of pectins and their role in vivo. We have isolated two allelic mutants, named quasimodo1 (qua1-1 and qua1-2), that are dwarfed and show reduced cell adhesion. Mutant cell walls showed a 25% reduction in galacturonic acid levels compared with the wild type, indicating reduced pectin content, whereas neutral sugars remained unchanged. Immersion immunofluorescence with the JIM5 and JIM7 monoclonal antibodies that recognize homogalacturonan epitopes revealed less labeling of mutant roots compared with the wild type. Both mutants carry a T-DNA insertion in a gene (QUA1) that encodes a putative membrane-bound glycosyltransferase of family 8. We present evidence for the possible involvement of a glycosyltransferase of this family in the synthesis of pectic polysaccharides, suggesting that other members of this large multigene family in Arabidopsis also may be important for pectin biosynthesis. The mutant phenotype is consistent with a central role for pectins in cell adhesion. |
| تدمد: | 1532-298X 1040-4651 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::285d751cb7086eb819c64642a1c1d83bTest https://doi.org/10.1105/tpc.004259Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....285d751cb7086eb819c64642a1c1d83b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1532298X 10404651 |
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