Nitromation of amines by stimulated macrophages
| العنوان: | Nitromation of amines by stimulated macrophages |
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| المؤلفون: | Misao Miwa, John S. Wishnok, Steven R. Tannenbaum, Dennis J. Stuehr, Michael A. Marletta |
| المصدر: | Carcinogenesis. 8:955-958 |
| بيانات النشر: | Oxford University Press (OUP), 1987. |
| سنة النشر: | 1987 |
| مصطلحات موضوعية: | Lipopolysaccharides, Mice, Inbred C3H, Cancer Research, Nitrates, Nitrosamines, Lipopolysaccharide, medicine.drug_class, Macrophages, General Medicine, Immunostimulant, In vitro, Cell Line, Mice, chemistry.chemical_compound, chemistry, Biochemistry, Nitrosamine, In vivo, Cell culture, Morpholine, medicine, Animals, Interferons, Nitrite, Nitrites |
| الوصف: | Rats and mice treated in vivo with Escherichia coli lipopolysaccharide (LPS) synthesize and excrete large quantities of nitrate. Murine peritoneal macrophages, elicited in vivo with thioglycolate and stimulated in vitro with LPS and/or gamma-interferon (IFN), produce copious amounts of nitrate and nitrite. We report here experiments showing N-nitrosamine formation by macrophages immunostimulated in vitro. Macrophage cell lines J774.1, PU5-1.8, WEHI-3 and RAW 264 and freshly isolated macrophages from C3H/He mice were used. Macrophages were cultured in Dulbecco's modified Eagle's medium (pH 7.5) supplemented with calf serum (10%). Supernatant NO2- and NO3- were measured. N-Nitrosamines were extracted with dichloromethane and the extracts analyzed by a gas chromatography--thermal energy analyzer. Cells (1.5 X 10(6)/ml) were incubated with LPS (10 micrograms/ml) and morpholine (15 mM) for 72 h at 37 degrees C. Under these conditions, all of the cell types listed above produced nitrite (40-70 microM) and N-nitrosomorpholine (NMOR; 114-940 nM). LPS was required for both processes, and this effect was enhanced by IFN. Nitrite (150 microM) incubated with morpholine in cell-free medium did not form NMOR nor did cells plus morpholine and NO2-. The rate of NMOR formation in the J774.1 cell line was highest in the middle incubation period (24-36 h) although [NO2-] was highest in the final incubation period (48-72 h). Thus, the cells do not catalyze nitrosamine formation per se, rather the amine traps out a reactive nitrosating species prior to the formation of NO2- and NO3-. These results suggest that immunostimulated macrophages may be capable of nitrosamine formation under physiological conditions. |
| تدمد: | 1460-2180 0143-3334 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8817817728ad4e96e9efd60d212bb368Test https://doi.org/10.1093/carcin/8.7.955Test |
| رقم الانضمام: | edsair.doi.dedup.....8817817728ad4e96e9efd60d212bb368 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602180 01433334 |
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