A Sensitive Liquid Chromatography-Mass Spectrometry Method for Determination of Toosendanin in Rat Plasma and its Application to Pharmacokinetic Study
| العنوان: | A Sensitive Liquid Chromatography-Mass Spectrometry Method for Determination of Toosendanin in Rat Plasma and its Application to Pharmacokinetic Study |
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| المؤلفون: | Chuangpeng Shen, Zhisen Pan, Xiaojie Wu, Chong Zhong, Qiao Li, Yuqi Si, Changhui Liu, Haitao Tu, Zhijun Deng, Zhangzhi Zhu, Jiewen Guo, Xiaoyi Xin, Min Liu |
| المصدر: | Journal of Chromatographic Science. 60:478-485 |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Rats, Sprague-Dawley, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Animals, Reproducibility of Results, General Medicine, Chromatography, High Pressure Liquid, Triterpenes, Chromatography, Liquid, Rats, Analytical Chemistry |
| الوصف: | A simple, rapid and sensitive analytical method was developed for the determination of toosendanin in rat plasma using liquid chromatography tandem mass spectrometry (LC–MS/MS). Andrographolide was selected as the internal standard, and the plasma samples were extracted by liquid–liquid extraction with diethyl ether. Chromatographic separation was performed on a Dikma Spursil C18, 3.5 μm (150 × 2.1 mm i.d) analytical column with 85% methanol:water (v/v) containing 0.025% formic acid (pH = 3.9) as mobile phase. The flow rate was 0.25 mL/min, and the total run time was 3 min. Detection was performed with a triple-quadrupole tandem mass spectrometer using negative ion mode electrospray ionization (ESI) in the multiple reaction monitoring (MRM) mode. The MS/MS ion transitions monitored were m/z 573.1 → 531.1 and 349.0 → 287.0 for toosendanin and andrographolide, respectively. Good linearity was observed over the concentration range of 3.125–500 ng/mL in 100 μL of rat plasma with a correlation coefficient ˃0.9997. Intra- and inter-assay variabilities were ˂8.5% in plasma. The recovery and the matrix effect were in the range 71.8–73.5% and 96.4–103.8%, respectively. The analyte was stable under various conditions (at room temperature, during freeze–thaw settings, in the autosampler, and under deep-freeze conditions). The method was successfully applied to a pharmacokinetic study of toosendanin after its oral administration in rats at a dose of 10 mg/kg. |
| تدمد: | 1945-239X 0021-9665 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8df568bbe6ea6ee02fc2f8f8c4b1ca86Test https://doi.org/10.1093/chromsci/bmab135Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8df568bbe6ea6ee02fc2f8f8c4b1ca86 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1945239X 00219665 |
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