A Proteomic Survey of Chlamydomonas reinhardtii Mitochondria Sheds New Light on the Metabolic Plasticity of the Organelle and on the Nature of the -Proteobacterial Mitochondrial Ancestor
| العنوان: | A Proteomic Survey of Chlamydomonas reinhardtii Mitochondria Sheds New Light on the Metabolic Plasticity of the Organelle and on the Nature of the -Proteobacterial Mitochondrial Ancestor |
|---|---|
| المؤلفون: | Brigitte Gontero, Oliver Deusch, Jérôme Garin, Marianne Tardif, Norbert Rolland, Sabine Brugière, Tal Dagan, Jacques Joyard, William Martin, Annie Adrait, Robert van Lis, Lauriane Kuhn, Ariane Atteia |
| المساهمون: | Laboratoire de physiologie cellulaire végétale (LPCV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'étude de la dynamique des protéomes (LEDyP), Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute of Botany, Heinrich-Heine-Universität Düsseldorf [Düsseldorf], Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Heinrich Heine Universität Düsseldorf = Heinrich Heine University [Düsseldorf], Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA) |
| المصدر: | Molecular Biology and Evolution Molecular Biology and Evolution, Oxford University Press (OUP), 2009, 26, pp.1533-1548. ⟨10.1093/molbev/msp068⟩ Molecular Biology and Evolution, 2009, 26, pp.1533-1548. ⟨10.1093/molbev/msp068⟩ |
| بيانات النشر: | Oxford University Press (OUP), 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | 0106 biological sciences, carbon metabolism, Algae, proteome, Chlamydomonas reinhardtii, nucleotide metabolism, Mitochondrion, phylogeny, 01 natural sciences, Genome, Oxidative Phosphorylation, 03 medical and health sciences, evolution, Organelle, Genetics, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Alphaproteobacteria, 030304 developmental biology, 0303 health sciences, biology, Chlamydomonas, metabolic pathway, sequence homology, bioinformatics, Vitamin biosynthesis, biology.organism_classification, Biological Evolution, Cell biology, mitochondria, enzyme, vitamin biosynthesis, targeting prediction, Proteome, Eukaryote, protein import, protein, metabolism, 010606 plant biology & botany |
| الوصف: | Mitochondria play a key role in the life and death of eukaryotic cells, yet the full spectrum of mitochondrial functions is far from being fully understood, especially in photosynthetic organisms. To advance our understanding of mitochondrial functions in a photosynthetic cell, an extensive proteomic survey of Percoll-purified mitochondria from the metabolically versatile, hydrogen-producing green alga Chlamydomonas reinhardtii was performed. Different fractions of purified mitochondria from Chlamydomonas cells grown under aerobic conditions were analyzed by nano-liquid chromatography-electrospray ionization-mass spectrometry after protein separation on sodium dodecyl sulfate polyacrylamide gel electrophoresis or on blue-native polyacrylamide gel electrophoresis. Of the 496 nonredundant proteins identified, 149 are known or predicted to reside in other cellular compartments and were thus excluded from the molecular and evolutionary analyses of the Chlamydomonas proteome. The mitochondrial proteome of the photosynthetic alga reveals important lineage-specific differences with other mitochondrial proteomes, reflecting the high metabolic diversity of the organelle. Some mitochondrial metabolic pathways in Chlamydomonas appear to combine typical mitochondrial enzymes and bacterial-type ones, whereas others are unknown among mitochondriate eukaryotes. The comparison of the Chlamydomonas proteins to their identifiable homologs predicted from 354 sequenced genomes indicated that Arabidopsis is the most closely related nonalgal eukaryote. Furthermore, this phylogenomic analysis shows that free-living alpha-proteobacteria from the metabolically versatile orders Rhizobiales and Rhodobacterales better reflect the gene content of the ancestor of the chlorophyte mitochondria than parasitic alpha-proteobacteria with reduced and specialized genomes. |
| تدمد: | 1537-1719 0737-4038 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b733bf3c0d307ea3b5d2fe22d628dd7Test https://doi.org/10.1093/molbev/msp068Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0b733bf3c0d307ea3b5d2fe22d628dd7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15371719 07374038 |
|---|