Signaling through Gαi2 protein is required for recruitment of neutrophils for antibody-mediated elimination of larval Strongyloides stercoralis in mice
| العنوان: | Signaling through Gαi2 protein is required for recruitment of neutrophils for antibody-mediated elimination of larval Strongyloides stercoralis in mice |
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| المؤلفون: | Kevin M. Redding, James J. Lee, Udaikumar M. Padigel, Lutz Birnbaumer, David Abraham, Gerhard A. Schad, Thomas J. Nolan, Louis H. Stein |
| المصدر: | Journal of Leukocyte Biology. 81:1120-1126 |
| بيانات النشر: | Oxford University Press (OUP), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Male, Neutrophils, medicine.medical_treatment, T cell, Immunology, Antibodies, Helminth, Article, Host-Parasite Interactions, Strongyloides stercoralis, Mice, Immune system, medicine, Animals, Immunology and Allergy, Cells, Cultured, B cell, biology, Cell Biology, biology.organism_classification, Immunity, Innate, Cytokine, medicine.anatomical_structure, Larva, Myeloperoxidase, Strongyloidiasis, biology.protein, GTP-Binding Protein alpha Subunit, Gi2, Signal transduction, Antibody, Signal Transduction |
| الوصف: | The heterotrimeric guanine nucleotide-binding protein Gαi2 is involved in regulation of immune responses against microbial and nonmicrobial stimuli. Gαi2−/− mice have a selectively impaired IgM response consistent with a disorder in B cell development yet have augmented T cell effector function associated with increased production of IFN-γ and IL-4. The goal of the present study was to determine if a deficiency in the Gαi2 protein in mice would affect the protective immune response against Strongyloides stercoralis, which is IL-4-, IL-5-, and IgM-dependent. Gαi2−/− and wild-type mice were immunized and challenged with S. stercoralis larvae and analyzed for protective immune responses against infection. Gαi2−/− mice failed to kill the larvae in the challenge infection as compared with wild-type mice despite developing an antigen-specific Th2 response characterized by increased IL-4, IL-5, IgM, and IgG. Transfer of serum collected from immunized Gαi2−/− mice to naïve wild-type mice conferred passive protective immunity against S. stercoralis infection thus confirming the development of a protective antibody response in Gαi2−/− mice. Differential cell analyses and myeloperoxidase assays for quantification of neutrophils showed a significantly reduced recruitment of neutrophils into the microenvironment of the parasites in immunized Gαi2−/− mice. However, cell transfer studies demonstrated that neutrophils from Gαi2−/− mice are competent in killing larvae. These data demonstrate that Gαi2 signaling events are not required for the development of the protective immune responses against S. stercoralis; however, Gαi2 is essential for the recruitment of neutrophils required for host-dependent killing of larvae. |
| تدمد: | 1938-3673 0741-5400 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90bef174ea37ce1ddcfeba4c811f43edTest https://doi.org/10.1189/jlb.1106695Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....90bef174ea37ce1ddcfeba4c811f43ed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19383673 07415400 |
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