دورية أكاديمية
A Granulocytic Signature Identifies COVID-19 and Its Severity
| العنوان: | A Granulocytic Signature Identifies COVID-19 and Its Severity |
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| المؤلفون: | Vitte, Joana, Diallo, Aïssatou Bailo, Boumaza, Asma, Lopez, Alexandre, Michel, Moïse, Allardet-Servent, Jérôme, Mezouar, Soraya, Sereme, Youssouf, Busnel, Jean-Marc, Miloud, Tewfik, Malergue, Fabrice, Morange, Pierre-Emmanuel, Halfon, Philippe, Olive, Daniel, Leone, Marc, Mege, Jean-Louis |
| المساهمون: | Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Aix-Marseille Université - École de médecine (AMU SMPM MED), Aix-Marseille Université - Faculté des sciences médicales et paramédicales (AMU SMPM), Aix Marseille Université (AMU)-Aix Marseille Université (AMU), CHU Marseille, Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Beckman Research Institute Duarte, CA, Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hôpital Européen Fondation Ambroise Paré - Marseille, Centre de Recherche en Cancérologie de Marseille (CRCM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU) |
| المصدر: | ISSN: 0022-1899. |
| بيانات النشر: | HAL CCSD Oxford University Press (OUP) |
| سنة النشر: | 2020 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | CD11b, CD16, PD-L1, immune checkpoint, CRTH2, basophil, eosinophil, neutrophil, COVID-19, SARS-CoV-2, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases |
| الوصف: | International audience ; Background An unbiased approach to SARS-CoV-2–induced immune dysregulation has not been undertaken so far. We aimed to identify previously unreported immune markers able to discriminate COVID-19 patients from healthy controls and to predict mild and severe disease.Methods An observational, prospective, multicentric study was conducted in patients with confirmed mild/moderate (n = 7) and severe (n = 19) COVID-19. Immunophenotyping of whole-blood leukocytes was performed in patients upon hospital ward or intensive care unit admission and in healthy controls (n = 25). Clinically relevant associations were identified through unsupervised analysis.Results Granulocytic (neutrophil, eosinophil, and basophil) markers were enriched during COVID-19 and discriminated between patients with mild and severe disease. Increased counts of CD15+CD16+ neutrophils, decreased granulocytic expression of integrin CD11b, and Th2-related CRTH2 downregulation in eosinophils and basophils established a COVID-19 signature. Severity was associated with emergence of PD-L1 checkpoint expression in basophils and eosinophils. This granulocytic signature was accompanied by monocyte and lymphocyte immunoparalysis. Correlation with validated clinical scores supported pathophysiological relevance.Conclusions Phenotypic markers of circulating granulocytes are strong discriminators between infected and uninfected individuals as well as between severity stages. COVID-19 alters the frequency and functional phenotypes of granulocyte subsets with emergence of CRTH2 as a disease biomarker. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/32941618; hal-03150816; https://hal-amu.archives-ouvertes.fr/hal-03150816Test; PUBMED: 32941618; WOS: 000605980700010 |
| DOI: | 10.1093/infdis/jiaa591 |
| الإتاحة: | https://doi.org/10.1093/infdis/jiaa591Test https://hal-amu.archives-ouvertes.fr/hal-03150816Test |
| رقم الانضمام: | edsbas.672403B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/infdis/jiaa591 |
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