Floral tissue ofPetunia hybrida(V30) expresses only one member of the chalcone synthase multigene family
| العنوان: | Floral tissue ofPetunia hybrida(V30) expresses only one member of the chalcone synthase multigene family |
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| المؤلفون: | H. J. Reif, Cornelis Spelt, E. Veltkamp, Ronald Koes, P.J.M. van den Elzen, J. N. M. Mol |
| المصدر: | Nucleic Acids Research. 14:5229-5239 |
| بيانات النشر: | Oxford University Press (OUP), 1986. |
| سنة النشر: | 1986 |
| مصطلحات موضوعية: | Chalcone synthase, Transcription, Genetic, Sequence analysis, Molecular cloning, Complementary DNA, Genetics, Coding region, Tissue Distribution, Amino Acid Sequence, Cloning, Molecular, skin and connective tissue diseases, Gene, Southern blot, Cloning, Base Sequence, integumentary system, biology, Chromosome Mapping, DNA, DNA Restriction Enzymes, Plants, Molecular biology, Gene Expression Regulation, Genes, biology.protein, Acyltransferases |
| الوصف: | Twenty independent, petal-specific chalcone synthase (CHS) cDNA clones have been isolated from Petunia hybrida variety Violet 30 (V30). Sequence analysis shows that the largest of these clones contains the entire coding sequence. Using this clone in Southern blot analysis reveals the presence of multiple CHS gene copies in the genome of Petunia hybrida V30. Hybridization and sequence analysis of the CHS cDNA clones shows that they are all copied from a single mRNA species. This indicates the presence of only one transcriptionally active CHS gene in petals. Finally we report the identification, cloning and partial characterization of this gene. |
| تدمد: | 1362-4962 0305-1048 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d3c4d4f7eb47a14c92be732d92a4054Test https://doi.org/10.1093/nar/14.13.5229Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4d3c4d4f7eb47a14c92be732d92a4054 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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