Isozyme-Specific Role of SAD-A in Neuronal Migration During Development of Cerebral Cortex
| العنوان: | Isozyme-Specific Role of SAD-A in Neuronal Migration During Development of Cerebral Cortex |
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| المؤلفون: | Yujiro Higashi, Shoichi Shimada, Madoka Hattori, Hiroyuki Niida, Kenichiro Yamada, Chisato Yamada, Hidenori Tabata, Kosei Takeuchi, Koh-ichi Nagata, Y. Albert Pan, Shinya Ugawa, Yoshikazu Johmura, Tsuyoshi Ito, Keiko Nakanishi, Joshua R. Sanes, Masashi Kishi, Takashi Ueda, Makoto Nakanishi, Yuki Hori, Nobuaki Wakamatsu |
| المصدر: | Cereb Cortex |
| بيانات النشر: | Oxford University Press (OUP), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, Neurite, Cognitive Neuroscience, Dendrite, Protein Serine-Threonine Kinases, Hippocampal formation, Biology, 050105 experimental psychology, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Cell Movement, medicine, Animals, 0501 psychology and cognitive sciences, Axon, Cells, Cultured, Cerebral Cortex, Mice, Knockout, Neurons, Kinase, Electroporation, 05 social sciences, Wild type, Axons, Cell biology, Isoenzymes, Mice, Inbred C57BL, medicine.anatomical_structure, nervous system, Cerebral cortex, Female, Original Article, 030217 neurology & neurosurgery |
| الوصف: | SAD kinases regulate presynaptic vesicle clustering and neuronal polarization. A previous report demonstrated that Sada−/− and Sadb−/− double-mutant mice showed perinatal lethality with a severe defect in axon/dendrite differentiation, but their single mutants did not. These results indicated that they were functionally redundant. Surprisingly, we show that on a C57BL/6N background, SAD-A is essential for cortical development whereas SAD-B is dispensable. Sada−/− mice died within a few days after birth. Their cortical lamination pattern was disorganized and radial migration of cortical neurons was perturbed. Birth date analyses with BrdU and in utero electroporation using pCAG-EGFP vector showed a delayed migration of cortical neurons to the pial surface in Sada−/− mice. Time-lapse imaging of these mice confirmed slow migration velocity in the cortical plate. While the neurites of hippocampal neurons in Sada−/− mice could ultimately differentiate in culture to form axons and dendrites, the average length of their axons was shorter than that of the wild type. Thus, analysis on a different genetic background than that used initially revealed a nonredundant role for SAD-A in neuronal migration and differentiation. |
| تدمد: | 1460-2199 1047-3211 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84e273655807b35031705c89564fdc1eTest https://doi.org/10.1093/cercor/bhy253Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....84e273655807b35031705c89564fdc1e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602199 10473211 |
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