دورية أكاديمية
Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.)
| العنوان: | Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.) |
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| المؤلفون: | Aranjuelo, Iker, Molero, Gemma, Erice, Gorka, Christophe Avice, Jean, Nogués, Salvador |
| المساهمون: | University of Barcelona, Universidad de Navarra Pamplona (UNAV), European Project PERMED INCO-CT-2004-509140, Spanish Science and Education Ministry BFU-2004-05096/BFI |
| المصدر: | ISSN: 0022-0957. |
| بيانات النشر: | HAL CCSD Oxford University Press (OUP) |
| سنة النشر: | 2011 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | Drought, Medicago sativa, N remobilization, oxidative stress, photosynthesis, proteome, Rubisco, RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE-OXYGENASE, WATER-WATER CYCLE, BRASSICA-NAPUS, XANTHOPHYLL CYCLE, AMINO-ACIDS, N(O)-TERT-BUTYLDIMETHYLSILYL DERIVATIVES, 2-CYSTEINE PEROXIREDOXIN, PINITOL ACCUMULATION, N-2 fixation, N-2 FIXATION RESPONSE, NITROGEN-FIXATION, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Despite its relevance, protein regulation, metabolic adjustment, and the physiological status of plants under drought is not well understood in relation to the role of nitrogen fixation in nodules. In this study, nodulated alfalfa plants were exposed to drought conditions. The study determined the physiological, metabolic, and proteomic processes involved in photosynthetic inhibition in relation to the decrease in nitrogenase (N-ase) activity. The deleterious effect of drought on alfalfa performance was targeted towards photosynthesis and N-ase activity. At the leaf level, photosynthetic inhibition was mainly caused by the inhibition of Rubisco. The proteomic profile and physiological measurements revealed that the reduced carboxylation capacity of droughted plants was related to limitations in Rubisco protein content, activation state, and RuBP regeneration. Drought also decreased amino acid content such as asparagine, and glutamic acid, and Rubisco protein content indicating that N availability limitations were caused by N-ase activity inhibition. In this context, drought induced the decrease in Rubisco binding protein content at the leaf level and proteases were up-regulated so as to degrade Rubisco protein. This degradation enabled the reallocation of the Rubisco-derived N to the synthesis of amino acids with osmoregulant capacity. Rubisco degradation under drought conditions was induced so as to remobilize Rubisco-derived N to compensate for the decrease in N associated with N-ase inhibition. Metabolic analyses showed that droughted plants increased amino acid (proline, a major compound involved in osmotic regulation) and soluble sugar (D-pinitol) levels to contribute towards the decrease in osmotic potential (Psi(s)). At the nodule level, drought had an inhibitory effect on N-ase activity. This decrease in N-ase activity was not induced by substrate shortage, as reflected by an increase in total soluble sugars (TSS) in the nodules. Proline accumulation in the nodule could also be ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-02647710; https://hal.inrae.fr/hal-02647710Test; PRODINRA: 206100; WOS: 000284951900010 |
| DOI: | 10.1093/jxb/erq249 |
| الإتاحة: | https://doi.org/10.1093/jxb/erq249Test https://hal.inrae.fr/hal-02647710Test |
| رقم الانضمام: | edsbas.26638D0E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/jxb/erq249 |
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