يعرض 1 - 7 نتائج من 7 نتيجة بحث عن '"quantitative rt-pcr"', وقت الاستعلام: 1.52s تنقيح النتائج
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    دورية أكاديمية
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    دورية أكاديمية

    المصدر: Sihto, Henna-Maria; Tasara, Taurai; Stephan, Roger; Johler, Sophia (2014). Validation of reference genes for normalization of qPCR mRNA expression levels in Staphylococcus aureus exposed to osmotic and lactic acid stress conditions encountered during food production and preservation. FEMS Microbiology Letters, 356(1):134-140.

    وصف الملف: application/pdf

    العلاقة: https://www.zora.uzh.ch/id/eprint/109440/1/356-1-134.pdfTest; info:pmid/24893820; urn:issn:0378-1097

  3. 3
    دورية أكاديمية

    المساهمون: Laboratoire de physiologie cellulaire végétale (LPCV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)

    المصدر: ISSN: 0032-0889.

    العلاقة: info:eu-repo/semantics/altIdentifier/pmid/19136569; hal-00370374; https://hal.science/hal-00370374Test; PRODINRA: 249861; PUBMED: 19136569; PUBMEDCENTRAL: PMC2649395; WOS: 000263843700005

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    دورية أكاديمية

    المصدر: Journal of Clinical Endocrinology and Metabolism; 92(6), pp 2292-2299 (2007) ; ISSN: 1945-7197

    مصطلحات موضوعية: Endocrinology and Diabetes, independent of fat mass. Data on weight reduction indicated that also other factors (e.g. leptin) relate to ATGL and HSL protein expression, we investigated whether a possible impaired expression could be reversed by a period of weight reduction. METHODS: Adipose tissue biopsies were taken from obese subjects (n = 44) with a wide range of insulin resistance, Obesity is associated with increased triacylglycerol (TAG) storage in adipose tissue and insulin resistance. The mobilization of stored TAG is mediated by hormone-sensitive lipase (HSL) and the recently discovered adipose triglyceride lipase (ATGL). The aim of the present study was to examine whether ATGL and HSL mRNA and protein expression are altered in insulin-resistant conditions. In addition, before and just after a 10-wk hypocaloric diet. ATGL and HSL protein and mRNA expression was determined by Western blot and quantitative RT-PCR, independent of age, gender, fat cell size, respectively. RESULTS: Fasting insulin levels and the degree of insulin resistance (using the homeostasis model assessment index for insulin resistance) were negatively correlated with ATGL and HSL protein expression, ATGL and HSL mRNA and protein levels seem to be highly correlated, and body composition. Both mRNA and protein levels of ATGL and HSL were reduced in insulin-resistant compared with insulin-sensitive subjects (P < 0.05). Weight reduction significantly decreased ATGL and HSL mRNA and protein expression. A positive correlation between the decrease in leptin and the decrease in ATGL protein level after weight reduction was observed. Finally, indicating a tight coregulation and transcriptional control. CONCLUSIONS: In obese subjects, insulin resistance and hyperinsulinemia are strongly associated with ATGL and HSL mRNA and protein expression

    العلاقة: https://lup.lub.lu.se/record/166621Test; http://dx.doi.org/10.1210/jc.2006-1318Test; wos:000247061700049; scopus:34347219137

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    دورية أكاديمية
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    دورية أكاديمية
  7. 7
    مورد إلكتروني

    المصدر: Sihto, Henna-Maria; Tasara, Taurai; Stephan, Roger; Johler, Sophia (2014). Validation of reference genes for normalization of qPCR mRNA expression levels in Staphylococcus aureus exposed to osmotic and lactic acid stress conditions encountered during food production and preservation. FEMS Microbiology Letters, 356(1):134-140.