دورية أكاديمية
Vitamin K2 Modulates Vitamin D‐Induced Mechanical Properties of Human 3D Bone Spheroids In Vitro
| العنوان: | Vitamin K2 Modulates Vitamin D‐Induced Mechanical Properties of Human 3D Bone Spheroids In Vitro |
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| المؤلفون: | Maria Schröder, Elisabeth Aurstad Riksen, Jianying He, Bjørn Helge Skallerud, Mona Elisabeth Møller, Aina‐Mari Lian, Unni Syversen, Janne Elin Reseland |
| المصدر: | JBMR Plus, Vol 4, Iss 9, Pp n/a-n/a (2020) |
| بيانات النشر: | Oxford University Press, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Orthopedic surgery LCC:Diseases of the musculoskeletal system |
| مصطلحات موضوعية: | BONE STIFFNESS, OSTEOBLASTS, OSTEOSPHERES, VITAMIN D, VITAMIN K2, Orthopedic surgery, RD701-811, Diseases of the musculoskeletal system, RC925-935 |
| الوصف: | ABSTRACT Rotational culture promotes primary human osteoblasts (hOBs) to form three‐dimensional (3D) multicellular spheroids with bone tissue‐like structure without any scaffolding material. Cell‐based bone models enable us to investigate the effect of different agents on the mechanical strength of bone. Given that low dietary intake of both vitamin D and K is negatively associated with fracture risk, we aimed to assess the effect of these vitamins in this system. Osteospheres of hOBs were generated with menaquinone‐4 (MK‐4; 10μM) and 25‐hydroxyvitamin D3 [25(OH)D3; 0.01μM], alone and in combination, or without vitamins. The mechanical properties were tested by nanoindentation using a flat‐punch compression method, and the mineralized extracellular bone matrix was characterized by microscopy. The in vitro response of hOBs to MK‐4 and 25(OH)D3 was further evaluated in two‐dimensional (2D) cultures and in the 3D bone constructs applying gene expression analysis and multiplex immunoassays. Mechanical testing revealed that 25(OH)D3 induced a stiffer and MK‐4 a softer or more flexible osteosphere compared with control. Combined vitamin conditions induced the same flexibility as MK‐4 alone. Enhanced levels of periostin (p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2473-4039 |
| العلاقة: | https://doaj.org/toc/2473-4039Test |
| DOI: | 10.1002/jbm4.10394 |
| الوصول الحر: | https://doaj.org/article/9ad3beb6f5014991a4b1d3e4be83404fTest |
| رقم الانضمام: | edsdoj.9ad3beb6f5014991a4b1d3e4be83404f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24734039 |
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| DOI: | 10.1002/jbm4.10394 |