Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variation
| العنوان: | Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variation |
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| المؤلفون: | Stefanie Stepanow, Karl Köhrer, Stefanie Kobus, Sonja Stallmann, Thomas Rattei, Johannes H. Hegemann, Thomas Eder |
| المصدر: | Pathogens and Disease |
| بيانات النشر: | Oxford University Press, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Sexually transmitted disease, Sexually Transmitted Diseases, Bacterial, 030106 microbiology, Chlamydia trachomatis, comparative genomics, Biology, medicine.disease_cause, Serogroup, Genome, DNA sequencing, 03 medical and health sciences, Young Adult, Bacterial Proteins, INDEL Mutation, Genetic variation, evolution, medicine, Immunology and Allergy, Humans, Chlamydia, Indel, Gene, genome, Genetics, Comparative genomics, General Immunology and Microbiology, Genetic Variation, General Medicine, Shortomics, Sequence Analysis, DNA, adhesins, Chlamydia Infections, 030104 developmental biology, Infectious Diseases, Female, Genome, Bacterial |
| الوصف: | Chlamydia trachomatis (Ctr) is a bacterial pathogen that causes ocular, urogenital and lymph system infections in humans. It is highly abundant and among its serovars, E, F and D are most prevalent in sexually transmitted disease. However, the number of publicly available genome sequences of the serovars E and F, and thereby our knowledge about the molecular architecture of these serovars, is low. Here we sequenced the genomes of six E and F clinical isolates and one E lab strain, in order to study the genetic variance in these serovars. As observed before, the genomic variation inside the Ctr genomes is very low and the phylogenetic placement in comparison to publicly available genomes is as expected by ompA gene serotyping. However, we observed a large InDel carrying four to five open reading frames in one clinical E sample and in the E lab strain. We have also observed substantial variation on nucleotide and amino acid levels, especially in membrane proteins and secreted proteins. Furthermore, these two groups of proteins are also target for recombination events. One clinical F isolate was genetically heterogeneous and revealed the highest differences on nucleotide level in the pmpE gene. Six Chlamydia trachomatis E and F clinical isolates were sequenced and analyzed, which are among the highly abundant and most prevalent serovars in sexually transmitted disease. |
| اللغة: | English |
| تدمد: | 2049-632X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::909e82d3ff5436c5706e5e27187d3d8cTest http://europepmc.org/articles/PMC5827700Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....909e82d3ff5436c5706e5e27187d3d8c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2049632X |
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