Infection Studies in Pigs and Porcine Airway Epithelial Cells Reveal an Evolution of A(H1N1)pdm09 Influenza A Viruses Toward Lower Virulence
| العنوان: | Infection Studies in Pigs and Porcine Airway Epithelial Cells Reveal an Evolution of A(H1N1)pdm09 Influenza A Viruses Toward Lower Virulence |
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| المؤلفون: | Yuguang Fu, Andi Krumbholz, Jie Tong, Ralf Dürrwald, Georg Herrler, Ludwig Haas, Ang Su, Xiangping Yin, Michaela Schmidtke, Nai-Huei Wu, Roland Zell, Fandan Meng |
| المصدر: | The Journal of Infectious Diseases |
| بيانات النشر: | Oxford University Press, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, viruses, Sus scrofa, Virulence, adaptation, Biology, medicine.disease_cause, Microbiology, 03 medical and health sciences, Major Articles and Brief Reports, 0302 clinical medicine, Influenza A Virus, H1N1 Subtype, Orthomyxoviridae Infections, In vivo, Suidae, evolution, medicine, Influenza A virus, Immunology and Allergy, Animals, 030212 general & internal medicine, differentiated airway epithelial cells, Lung, Cells, Cultured, Epithelial Cells, Viral Load, biology.organism_classification, Epithelium, In vitro, 3. Good health, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Viruses, pandemic H1N1 2009 influenza A viruses, Viral load |
| الوصف: | Investigations in pigs and porcine differentiated airway epithelial cells (PDAECs) reflect a loss in virulence of A(H1N1)pdm09 viruses isolated from humans and swine (2009–2015). PDAEC may be an in vitro test system for virulence of influenza A viruses. We analyzed the virulence of pandemic H1N1 2009 influenza A viruses in vivo and in vitro. Selected viruses isolated in 2009, 2010, 2014, and 2015 were assessed using an aerosol-mediated high-dose infection model for pigs as well as air-liquid interface cultures of differentiated airway epithelial cells. Using a dyspnea score, rectal temperature, lung lesions, and viral load in the lung as parameters, the strains from 2014–2015 were significantly less virulent than the strains isolated in 2009–2010. In vitro, the viruses from 2009–2010 also differed from the 2014–2015 viruses by increased release of infectious virus, a more pronounced loss of ciliated cells, and a reduced thickness of the epithelial cell layer. Our in vivo and in vitro results reveal an evolution of A(H1N1)pdm09 viruses toward lower virulence. Our in vitro culture system can be used to predict the virulence of influenza viruses. |
| اللغة: | English |
| تدمد: | 1537-6613 0022-1899 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::865420e5103501162780e587c09860d5Test http://europepmc.org/articles/PMC7107423Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....865420e5103501162780e587c09860d5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15376613 00221899 |
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